| IED ID | IndEnz0010000499 |
| Enzyme Type ID | esterase000499 |
| Protein Name |
Pectinesterase EC 3.1.1.11 Pectin methylesterase |
| Gene Name | CE8-1 |
| Organism | Sitophilus oryzae (Rice weevil) (Curculio oryzae) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Coleoptera Polyphaga Cucujiformia Curculionoidea Curculionidae (weevils) Dryophthorinae (palm weevils) Sitophilus Sitophilus oryzae (Rice weevil) (Curculio oryzae) |
| Enzyme Sequence | MKIIVLLLLAVVLASADQTAPGTASRPILTASESNYFTTATYLQGWSPPSISTSKADYTVGNGYNTIQAAVNAAINTGGTTRKYIKINAGTYQEVVYIPNTKVPLTIYGGGSSPSDTLITLNMPAQTTPSAYKSLVGSLFNSADPAYSMYNSCASKSGTIGTSCSTVFWVKAPAVQIVNLSIENSAKNTGDQQAVALQTNSDQIQIHNARLLGHQDTLYAGSGSSSVERSYYTNTYIEGDIDFVFGGGSAIFESCTFYVKADRRSDTAVVFAPDTDPHKMYGYFVYKSTITGDSAWSSSKKAYLGRAWDSGVSSSSAYVPGTSPNGQLIIKESTIDGIINTSGPWTTATSGRTYSGNNANSRDLNNDNYNRFWEYNNSGNGA |
| Enzyme Length | 382 |
| Uniprot Accession Number | E7CIP7 |
| Absorption | |
| Active Site | ACT_SITE 216; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P0C1A9; ACT_SITE 242; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P0C1A9 |
| Activity Regulation | |
| Binding Site | BINDING 193; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1A9; BINDING 306; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1A9; BINDING 308; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1A9 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; Evidence={ECO:0000269|PubMed:16341253}; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Pectinesterase which probably plays an important role in the digestion of plant cell walls. {ECO:0000305|PubMed:16341253}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. {ECO:0000269|PubMed:16341253}. |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (27); Binding site (3); Chain (1); Disulfide bond (1); Glycosylation (3); Helix (10); Sequence conflict (6); Signal peptide (1); Site (1); Turn (2) |
| Keywords | 3D-structure;Aspartyl esterase;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000305|PubMed:16341253 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 4PMH; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,697 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda |