IED ID | IndEnz0010000504 |
Enzyme Type ID | esterase000504 |
Protein Name |
Putative methylesterase 12, chloroplastic AtMES12 EC 3.1.1.- |
Gene Name | MES12 At4g09900/At4g09910 T5L19.30/T5L19.40 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MGNRVICMKKKDVVIRSGGDGSRSKRVNRSQRKLLADEENLHRRALSMAIHQAQVSQRFDGSMSRRIGSTSSRRGTLSDSFSNNKQVPEFLESLKVKKFVLVHGEGFGAWCWYKTIASLEESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACVSYALERFPEKISKAIFICATMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPATGFMFEKQHMKGLYFNQSPNKDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLDDLALSPDVQEKLVRENSPEAVFKIKGSDHCPFFSKPQSLHKILLEIAQIP |
Enzyme Length | 349 |
Uniprot Accession Number | Q940H7 |
Absorption | |
Active Site | ACT_SITE 173; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 300; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 328; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | 3.1.1.- |
Enzyme Function | FUNCTION: Putative methylesterase. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Chain (1); Erroneous gene model prediction (4); Region (1); Transit peptide (1) |
Keywords | Chloroplast;Hydrolase;Plastid;Reference proteome;Transit peptide |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 18643994; 18650403; |
Motif | |
Gene Encoded By | |
Mass | 38,930 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |