Detail Information for IndEnz0010000504
IED ID IndEnz0010000504
Enzyme Type ID esterase000504
Protein Name Putative methylesterase 12, chloroplastic
AtMES12
EC 3.1.1.-
Gene Name MES12 At4g09900/At4g09910 T5L19.30/T5L19.40
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MGNRVICMKKKDVVIRSGGDGSRSKRVNRSQRKLLADEENLHRRALSMAIHQAQVSQRFDGSMSRRIGSTSSRRGTLSDSFSNNKQVPEFLESLKVKKFVLVHGEGFGAWCWYKTIASLEESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACVSYALERFPEKISKAIFICATMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPATGFMFEKQHMKGLYFNQSPNKDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLDDLALSPDVQEKLVRENSPEAVFKIKGSDHCPFFSKPQSLHKILLEIAQIP
Enzyme Length 349
Uniprot Accession Number Q940H7
Absorption
Active Site ACT_SITE 173; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 300; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 328; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Putative methylesterase.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Erroneous gene model prediction (4); Region (1); Transit peptide (1)
Keywords Chloroplast;Hydrolase;Plastid;Reference proteome;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 18643994; 18650403;
Motif
Gene Encoded By
Mass 38,930
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda