| IED ID | IndEnz0010000545 |
| Enzyme Type ID | esterase000545 |
| Protein Name |
Lipase 2 EC 3.1.1.3 |
| Gene Name | LIP2 |
| Organism | Diutina rugosa (Yeast) (Candida rugosa) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetales incertae sedis Diutina Diutina rugosa (Yeast) (Candida rugosa) |
| Enzyme Sequence | MKLCLLALGAAVAAAPTATLANGDTITGLNAIVNEKFLGIPFAEPPVGTLRFKPPVPYSASLNGQQFTSYGPSCMQMNPMGSFEDTLPKNARHLVLQSKIFQVVLPNDEDCLTINVIRPPGTRASAGLPVMLWIFGGGFELGGSSLFPGDQMVAKSVLMGKPVIHVSMNYRVASWGFLAGPDIQNEGSGNAGLHDQRLAMQWVADNIAGFGGDPSKVTIYGESAGSMSTFVHLVWNDGDNTYNGKPLFRAAIMQSGCMVPSDPVDGTYGTEIYNQVVASAGCGSASDKLACLRGLSQDTLYQATSDTPGVLAYPSLRLSYLPRPDGTFITDDMYALVRDGKYAHVPVIIGDQNDEGTLFGLSSLNVTTDAQARAYFKQSFIHASDAEIDTLMAAYTSDITQGSPFDTGIFNAITPQFKRISALLGDLAFTLARRYFLNYYQGGTKYSFLSKQLSGLPVLGTFHGNDIIWQDYLVGSGSVIYNNAFIAFANDLDPNKAGLWTNWPTYTSSSQSGNNLMQINGLGLYTGKDNFRPDAYSALFSNPPSFFV |
| Enzyme Length | 548 |
| Uniprot Accession Number | P32946 |
| Absorption | |
| Active Site | ACT_SITE 223; /note=Acyl-ester intermediate; ACT_SITE 355; /note=Charge relay system; ACT_SITE 463; /note=Charge relay system |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (18); Chain (1); Disulfide bond (2); Glycosylation (1); Helix (27); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..14 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1GZ7; |
| Mapped Pubmed ID | 2506450; 9379937; |
| Motif | |
| Gene Encoded By | |
| Mass | 58,865 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda |