IED ID | IndEnz0010000651 |
Enzyme Type ID | esterase000651 |
Protein Name |
Methylesterase 10 AtMES10 EC 3.1.1.- |
Gene Name | MES10 At3g50440 T20E23_40 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MTYQKQYQMQTHHMQQQQLHHFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSFMESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTRLPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMALMKPSWLYTKEMGGEDLITKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRIEEAGHMAMLTKPHELSQLLQEIAAKYN |
Enzyme Length | 275 |
Uniprot Accession Number | Q8S9K8 |
Absorption | |
Active Site | ACT_SITE 96; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 225; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 253; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + methyl (-)-jasmonate = H(+) + jasmonate + methanol; Xref=Rhea:RHEA:55372, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15929, ChEBI:CHEBI:17790, ChEBI:CHEBI:58431; Evidence={ECO:0000269|PubMed:18467465};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55373; Evidence={ECO:0000269|PubMed:18467465}; |
DNA Binding | |
EC Number | 3.1.1.- |
Enzyme Function | FUNCTION: Methylesterase shown to have methyl jasmonate (MeJA) esterase activity in vitro. {ECO:0000269|PubMed:18467465}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Plant hormone biosynthesis. {ECO:0000269|PubMed:18467465}.; PATHWAY: Lipid metabolism; oxylipin biosynthesis. {ECO:0000269|PubMed:18467465}. |
nucleotide Binding | |
Features | Active site (3); Chain (1); Erroneous initiation (1) |
Keywords | Hydrolase;Reference proteome |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 15155802; 16081527; 16673935; 17122067; 18643994; 18650403; 21798944; 28627464; 31519798; |
Motif | |
Gene Encoded By | |
Mass | 31,358 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:55372; RHEA:55373 |
Cross Reference Brenda |