Detail Information for IndEnz0010000660
IED ID IndEnz0010000660
Enzyme Type ID esterase000660
Protein Name Probable carboxylesterase 4, mitochondrial
AtCXE4
EC 3.1.1.1
Gene Name CXE4 At1g49650 F14J22.21
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MLRRITCSSSLASPSLFLRFFRQLPRSYSSPTTIAVSGRNIRRLSTPTTLRCICSHSSSEIISEHPPFVRVYKDGRIERLSGTETVPASLNPRNDVVSKDVVYSPGHNLSVRLFLPHKSTQLAAGNKLPLLIYFHGGAWINESPFSPIYHNFLTEVVKSANCLAVSVQYRRAPEDPVPAAYEDTWSAIQWIFSHSCGSGEEDWINKYADFERVFLAGDSAGGNISHHMAMRAGKEKLKPRIKGTVIVHPAIWGKDPVDEHDVQDREIRDGVAEVWEKIVSPNSVDGADDPWFNVVGSGSNFSGMGCDKVLVEVAGKDVFWRQGLAYAAKLKKSGWKGEVEVIEEEDEEHCFHLLNPSSENAPSFMKRFVEFITG
Enzyme Length 374
Uniprot Accession Number Q9FX93
Absorption
Active Site ACT_SITE 219; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 317; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 349; /evidence=ECO:0000250|UniProtKB:Q5NUF3
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
DNA Binding
EC Number 3.1.1.1
Enzyme Function FUNCTION: Carboxylesterase acting on esters with varying acyl chain length. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Motif (1); Sequence conflict (7); Transit peptide (1)
Keywords Hydrolase;Mitochondrion;Reference proteome;Serine esterase;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305|PubMed:25732537}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 18805951; 28627464;
Motif MOTIF 135..137; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3
Gene Encoded By
Mass 41,693
Kinetics
Metal Binding
Rhea ID RHEA:21164
Cross Reference Brenda