| IED ID | IndEnz0010000689 |
| Enzyme Type ID | esterase000689 |
| Protein Name |
2-hydroxyisoflavanone dehydratase EC 3.1.1.1 EC 4.2.1.105 Carboxylesterase HIDM |
| Gene Name | HIDM |
| Organism | Glycyrrhiza echinata (Licorice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade Hologalegina IRL clade Galegeae Glycyrrhiza Glycyrrhiza echinata (Licorice) |
| Enzyme Sequence | MASSTSTTTSKEIDRELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTISARVYLPKLNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAALRVGAEALPGGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGIDNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGEEHCFQIYHPETENSKDLIGRIASFLV |
| Enzyme Length | 328 |
| Uniprot Accession Number | Q5NUF4 |
| Absorption | |
| Active Site | ACT_SITE 173; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 272; /evidence=ECO:0000250|UniProtKB:Q5NUF3; ACT_SITE 304; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(2R,3S)-2,4',7-trihydroxyisoflavanone = daidzein + H(+) + H2O; Xref=Rhea:RHEA:16445, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:63325, ChEBI:CHEBI:77764; EC=4.2.1.105; Evidence={ECO:0000269|PubMed:15734910}; CATALYTIC ACTIVITY: Reaction=2-hydroxy-2,3-dihydrogenistein = genistein + H(+) + H2O; Xref=Rhea:RHEA:36803, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:31080, ChEBI:CHEBI:74224; EC=4.2.1.105; Evidence={ECO:0000269|PubMed:15734910}; CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000269|PubMed:15734910}; |
| DNA Binding | |
| EC Number | 3.1.1.1; 4.2.1.105 |
| Enzyme Function | FUNCTION: Dehydratase that mediates the biosynthesis of isoflavonoids. Can better use 2,7-dihydroxy-4'-methoxyisoflavanone as substrate. Has also a slight carboxylesterase activity toward p-nitrophenyl butyrate. {ECO:0000269|PubMed:15734910}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Motif (1) |
| Keywords | Flavonoid biosynthesis;Hydrolase;Lyase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 85..87; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
| Gene Encoded By | |
| Mass | 36,061 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=58 uM for 2,7-dihydroxy-4'-methoxyisoflavanone (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:15734910}; KM=210 uM for 2,7,4'-trihydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:15734910}; KM=304 uM for 2,5,7,4'-tetrahydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:15734910}; Note=kcat is 9.8 sec(-1) with 2,7-dihydroxy-4'-methoxyisoflavanone, 0.12 sec(-1) with 2,7,4'-trihydroxyisoflavanone and 0.19 sec(-1) with 2,5,7,4'-tetrahydroxyisoflavanone as substrates, respectively (at pH 7.5 and 30 degrees Celsius).; |
| Metal Binding | |
| Rhea ID | RHEA:16445; RHEA:36803; RHEA:21164 |
| Cross Reference Brenda | 4.2.1.105; |