Detail Information for IndEnz0010000696
IED ID IndEnz0010000696
Enzyme Type ID esterase000696
Protein Name Platelet-activating factor acetylhydrolase IB subunit beta
Lissencephaly-1 protein
LIS-1
PAF acetylhydrolase 45 kDa subunit
PAF-AH 45 kDa subunit
PAF-AH alpha
PAFAH alpha
Gene Name Pafah1b1 Lis-1 Lis1 Pafaha
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR
Enzyme Length 410
Uniprot Accession Number P63005
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Regulatory subunit (beta subunit) of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)), an enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and participates in PAF inactivation. Regulates the PAF-AH (I) activity in a catalytic dimer composition-dependent manner (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos (By similarity). May modulate the Reelin pathway through interaction of the PAF-AH (I) catalytic dimer with VLDLR (PubMed:17330141). {ECO:0000250|UniProtKB:P43033, ECO:0000250|UniProtKB:P43034, ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:11056530, ECO:0000269|PubMed:11344260, ECO:0000269|PubMed:12796778, ECO:0000269|PubMed:12911752, ECO:0000269|PubMed:14507966, ECO:0000269|PubMed:14578885, ECO:0000269|PubMed:14691133, ECO:0000269|PubMed:15173193, ECO:0000269|PubMed:15473966, ECO:0000269|PubMed:16107726, ECO:0000269|PubMed:16203747, ECO:0000269|PubMed:16369480, ECO:0000269|PubMed:16481446, ECO:0000269|PubMed:17330141}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Alternative sequence (1); Beta strand (30); Chain (1); Coiled coil (1); Domain (1); Helix (6); Modified residue (2); Mutagenesis (9); Region (6); Repeat (7); Turn (5)
Keywords 3D-structure;Acetylation;Alternative splicing;Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;Direct protein sequencing;Lipid degradation;Lipid metabolism;Membrane;Microtubule;Mitosis;Neurogenesis;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transport;WD repeat
Interact With Q9ERR1; Q61205; Q9GZM8
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Nucleus membrane {ECO:0000255|HAMAP-Rule:MF_03141}. Note=May localize to the nuclear membrane (By similarity). Localizes to the plus end of microtubules and to the centrosome. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes. {ECO:0000250}.
Modified Residue MOD_RES 53; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:P43034; MOD_RES 109; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P43034
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 1UUJ; 1VYH;
Mapped Pubmed ID 10026115; 10371146; 10393212; 10403203; 10491172; 10541472; 10729324; 11159375; 11172053; 11210178; 11803439; 11911856; 12130536; 12200366; 12520002; 12629176; 12775763; 12904583; 12905154; 13129914; 14610273; 14681479; 15208636; 15331402; 15762842; 15998744; 16141072; 16172262; 16445939; 16602821; 16615898; 17130546; 17148952; 17314247; 17433713; 17555748; 17881479; 17903175; 17967808; 17997972; 18075262; 18075263; 18267077; 18331715; 18434304; 18446829; 18469343; 18514414; 18524253; 18799693; 18809722; 18983980; 19515951; 19734909; 19861636; 19874786; 19938147; 20007476; 20181597; 20360068; 20364144; 20531249; 20620871; 20624590; 21092859; 21183738; 21267068; 21350014; 21423666; 21515572; 21734362; 21844209; 21911489; 21935943; 22028625; 22073305; 22114287; 22522921; 22973010; 23483698; 23483716; 23533177; 23673330; 23783758; 23830831; 23926272; 24030547; 24487275; 24812355; 24853954; 24952961; 25245017; 25698733; 26079645; 26098214; 26496610; 26834590; 27029586; 2740347; 27994513; 28039900; 28446568; 28630356; 28836069; 29150182; 29404402; 29470990; 29475944; 29562183; 30923192; 31027966; 31073041; 31474834; 33150866; 7767001; 8619131; 8954729; 9199935; 9601647; 9623676; 9697693;
Motif
Gene Encoded By
Mass 46,670
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda