| IED ID | IndEnz0010000703 |
| Enzyme Type ID | esterase000703 |
| Protein Name |
Ethanol acetyltransferase 1 EC 2.3.1.268 Acetyl-CoA hydrolase EC 3.1.2.1 Acetyl-CoA thioesterase Alcohol acetyltransferase AAT Ethyl acetate esterase EC 3.1.1.- |
| Gene Name | EAT1 WICANDRAFT_27004 |
| Organism | Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8) (Yeast) (Hansenula anomala) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Phaffomycetaceae Wickerhamomyces Wickerhamomyces anomalus (Yeast) (Hansenula anomala) Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8) (Yeast) (Hansenula anomala) |
| Enzyme Sequence | MFFTKVLNNQVANGLKQLPVHKRVQMAYDLHIPNKTVNPNLNIRSHEPIVFVHGIFGSKKNYRHDCQKIANVTHTPVYTIDLRNHGQSMHALPFDYETLAQDVTDFCEDHGLKKVNLIGYSLGAKICMLTMLQNPDLVRSGVIIDNSPIEQPHIEIFLTQFIKSMLHVLNSTKIRADDKDWKSKANQAMRRYIPNGGIRDYLLANLINKVPKGYKSPVINYDDGYIHFQNPVRHMTEVAVKNVSAWPTEHVKGLKFEGQVRFLKGTKSAFIDEKGLEAIKEYFPNYSLSELNATHFILNERPQEYVKLICDFIKVNRYKSLQEHIRHVENFSSAELEARHNAEHERQMEELRQLTQTTPTAEQVKTIDNLASKVDLSATERQQQQNKEITV |
| Enzyme Length | 391 |
| Uniprot Accession Number | A0A1E3P8S6 |
| Absorption | |
| Active Site | ACT_SITE 121; /note=Charge relay system; /evidence=ECO:0000305|PubMed:28356220; ACT_SITE 145; /note=Charge relay system; /evidence=ECO:0000305|PubMed:28356220; ACT_SITE 295; /note=Charge relay system; /evidence=ECO:0000305|PubMed:28356220 |
| Activity Regulation | ACTIVITY REGULATION: By ethanol. Thioesterase and esterase reactions are highly repressed in the presence of high ethanol concentrations. {ECO:0000269|PubMed:28356220}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetyl-CoA + ethanol = CoA + ethyl acetate; Xref=Rhea:RHEA:55972, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.268; Evidence={ECO:0000269|PubMed:28356220}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1; Evidence={ECO:0000269|PubMed:28356220}; CATALYTIC ACTIVITY: Reaction=ethyl acetate + H2O = acetate + ethanol + H(+); Xref=Rhea:RHEA:58148, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750, ChEBI:CHEBI:30089; Evidence={ECO:0000269|PubMed:28356220}; |
| DNA Binding | |
| EC Number | 2.3.1.268; 3.1.2.1; 3.1.1.- |
| Enzyme Function | FUNCTION: Alcohol acetyltransferase that catalyzes the synthesis of ethyl acetate from ethanol and acetyl-CoA. Can also function as a thioesterase by hydrolyzing acetyl-CoA in the absence of ethanol, as well as esterase hydrolyzing ethyl acetate. {ECO:0000269|PubMed:28356220}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Transit peptide (1) |
| Keywords | Coiled coil;Hydrolase;Mitochondrion;Reference proteome;Transferase;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 45,069 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.12 mM for acetyl-CoA (for acetyl-CoA hydrolase reaction) {ECO:0000269|PubMed:28356220}; KM=2.43 mM for ethanol (for alcohol acetyltransferase reaction) {ECO:0000269|PubMed:28356220}; |
| Metal Binding | |
| Rhea ID | RHEA:55972; RHEA:20289; RHEA:58148 |
| Cross Reference Brenda | 2.3.1.268; |