| IED ID | IndEnz0010000724 |
| Enzyme Type ID | esterase000724 |
| Protein Name |
2-hydroxymuconate semialdehyde hydrolase HMSH EC 3.7.1.9 2-hydroxymuconic semialdehyde hydrolase |
| Gene Name | dmpD |
| Organism | Pseudomonas sp. (strain CF600) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Proteobacteria incertae sedis unclassified pseudomonads Pseudomonas sp. (strain CF600) |
| Enzyme Sequence | MNAPQNSPEIGREIIAAGIRTNLHDSGAGFPLMMIHGSGPGVTAWANWRLVMPELAKSRRVIAPDMLGFGYSERPADAQYNRDVWVDHAVGVLDALEIEQADLVGNSFGGGIALALAIRHPERVRRLVLMGSAGVSFPITEGLDAVWGYNPSFAEMRRLLDIFAFDRNLVNDELAELRYQASIRPGFHESFAAMFPAPRQRWVDGLASAEAAIRALPHETLVIHGREDQIIPLQTSLTLADWIARAQLHVFGQCGHWTQIEHAARFASLVGDFLAEADAAAIS |
| Enzyme Length | 283 |
| Uniprot Accession Number | P19076 |
| Absorption | |
| Active Site | ACT_SITE 107; /evidence=ECO:0000250; ACT_SITE 228; /evidence=ECO:0000250; ACT_SITE 256; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O = 2-oxopent-4-enoate + formate + H(+); Xref=Rhea:RHEA:14549, ChEBI:CHEBI:11641, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:71198; EC=3.7.1.9; |
| DNA Binding | |
| EC Number | 3.7.1.9 |
| Enzyme Function | FUNCTION: Catalyzes the conversion of 2-hydroxymuconate semialdehyde to 2-hydroxypent-2,4-dienoate. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Aromatic compound metabolism; benzoate degradation via hydroxylation. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1) |
| Keywords | Aromatic hydrocarbons catabolism;Hydrolase;Plasmid;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | Plasmid pVI150 |
| Mass | 30,965 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:14549 |
| Cross Reference Brenda |