| IED ID | IndEnz0010000729 |
| Enzyme Type ID | esterase000729 |
| Protein Name |
Palmitoleoyl-protein carboxylesterase NOTUM EC 3.1.1.98 Protein Notum Protein wingful dNOTUM |
| Gene Name | Notum wf CG13076 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MAVEQIDKMAAKAGEATNKWIKPQQPLLTLLLLLATFSQLPAVCSSSILDAASLQEKDPLRDTSMNMIQRNYMVMHSASGSGDHSRSLKRANLANTSITCNDGSHAGFYLRKHPSSKKWIVLLEGGWHCFDVRSCRSRWMRLRHLMTSSQWPETRDVGGILSPHPEENPYWHNANHVLIPYCSSDSWSGTRTEPDTSDRENSWRFMGALILRQVIAELIPVGLGRVPGGELMLVGSSAGGMGVMLNLDRIRDFLVNEKKLQITVRGVSDSGWFLDREPYTPAAVASNEAVRQGWKLWQGLLPEECTKSYPTEPWRCYYGYRLYPTLKTPLFVFQWLFDEAQMRVDNVGAPVTPQQWNYIHEMGGALRSSLDNVSAVFAPSCIGHGVLFKRDWVNIKIDDISLPSALRCWEHSTRSRRHDKLKRSTEPSTAVSHPEHANNQRHQRHRQRLQRQKHNNVAQSGGQQRKHNHLSKEEREERKRLRQEQRQRRKQRRRQQQQKKANGGQEHRNKKDNSPKSSNGNDQRKQRRRQQLTAEERQEQRKRRRKAQQQQMKMQREQPAAGVFLEASAPQKTRSSNNASAGTKSKKRHRVPRVPEKCGLRLLERCSWPQCNHSCPTLTNPMTGEEMRFLELLTAFGLDIEAVAAALGVDMHTLNNMERTELVNMLTQQAN |
| Enzyme Length | 671 |
| Uniprot Accession Number | Q9VUX3 |
| Absorption | |
| Active Site | ACT_SITE 237; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 338; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 384; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + H2O = (9Z)-hexadecenoate + [Wnt protein]-L-serine + H(+); Xref=Rhea:RHEA:45340, Rhea:RHEA-COMP:11170, Rhea:RHEA-COMP:11171, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:32372, ChEBI:CHEBI:85189; EC=3.1.1.98; Evidence={ECO:0000250|UniProtKB:Q6P988}; |
| DNA Binding | |
| EC Number | 3.1.1.98 |
| Enzyme Function | FUNCTION: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175). Also acts as a regulator of long-range activity of Hedgehog (hh), possibly by regulating the switch between low and high level hh pathway signaling (PubMed:20412775, PubMed:22872085). {ECO:0000250|UniProtKB:Q6P988, ECO:0000269|PubMed:20412775, ECO:0000269|PubMed:22872085, ECO:0000269|PubMed:25731175}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (14); Chain (1); Compositional bias (3); Glycosylation (4); Helix (15); Mutagenesis (1); Region (1); Signal peptide (1); Turn (4) |
| Keywords | 3D-structure;Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal;Wnt signaling pathway |
| Interact With | |
| Induction | INDUCTION: Expression is induced by Wnt. {ECO:0000269|PubMed:12000788, ECO:0000269|PubMed:12015973}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12000788, ECO:0000269|PubMed:12015973, ECO:0000269|PubMed:25731175}. Cell surface {ECO:0000269|PubMed:25731175}. Note=Associates with the cell surface via interaction with sulfated CAG chains on glypicans. {ECO:0000269|PubMed:25731175}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..46; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 4UZJ; 4UZK; |
| Mapped Pubmed ID | 11779831; 14660542; 14757636; 15148297; 15469840; 15494302; 15531366; 15563523; 15601817; 15647319; 15834138; 16041759; 16364628; 16914498; 17410209; 17967162; 17989227; 18160704; 18418383; 18477454; 18786525; 18824696; 18987026; 19062282; 19167331; 19178699; 20203130; 20220848; 20371351; 20422447; 20439429; 20932848; 21074052; 21177962; 21305649; 21483663; 21839923; 21853143; 21858158; 22115527; 22152945; 22192913; 23071443; 23509066; 23583758; 23868318; 24131631; 24412155; 24675402; 25074684; 25242144; 25294943; 26092715; 26173873; 26839340; 26907691; 26936649; 26974662; 27172210; 27269283; 28030561; 28299188; 28708826; 28860114; 29431244; 29618590; 30683884; 31194729; 32024666; 32699409; 32788209; 32901612; 34252547; 34343293; 34389661; 34580289; 8601490; |
| Motif | |
| Gene Encoded By | |
| Mass | 76,937 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:45340 |
| Cross Reference Brenda | 3.1.1.98; |