| IED ID | IndEnz0010000748 |
| Enzyme Type ID | esterase000748 |
| Protein Name |
Pectinesterase 1 PE 1 EC 3.1.1.11 Pectin methylesterase 1 AtPME1 |
| Gene Name | PME1 ARATH65 At1g53840 T18A20.7 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPIHRRRRLMSHHHQQSVDFEKWARRRLLQTAGLKPDVTVAGDGTGDVLTVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGVINQLS |
| Enzyme Length | 586 |
| Uniprot Accession Number | Q43867 |
| Absorption | |
| Active Site | ACT_SITE 408; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 429; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040 |
| Activity Regulation | |
| Binding Site | BINDING 355; /note=Substrate; /evidence=ECO:0000250; BINDING 385; /note=Substrate; /evidence=ECO:0000250; BINDING 492; /note=Substrate; /evidence=ECO:0000250; BINDING 494; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin (By similarity). Demethylates protein phosphatase 2A (PP2A) that have been reversibly carboxymethylated by LCMT1. Acts as negative regulators of genes involved in salt stress response (PubMed:28741704). {ECO:0000250|UniProtKB:Q5MFV8, ECO:0000269|PubMed:28741704}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Disulfide bond (1); Glycosylation (6); Motif (2); Signal peptide (1); Site (1) |
| Keywords | Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Reference proteome;Secreted;Signal;Stress response |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:19144003}. Golgi apparatus membrane {ECO:0000269|PubMed:19144003}. Note=Cleaved in the Golgi apparatus by SBT6.1 (S1P) after the Arg-Arg-Leu-Met (RRLM) and Arg-Arg-Leu-Leu (RRLL) motifs. This processing is required for extracellular targeting. {ECO:0000269|PubMed:19144003}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..49; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12169696; 12671089; 14996219; 16621846; 17149585; 17151019; 17317660; 17432890; 17644812; 17660356; 18650403; 18805951; 19001564; 21245191; 25122472; 28082716; 31519798; |
| Motif | MOTIF 250..253; /note=RRLM cleavage motif; /evidence=ECO:0000305|PubMed:19144003; MOTIF 269..272; /note=RRLL cleavage motif; /evidence=ECO:0000305|PubMed:19144003 |
| Gene Encoded By | |
| Mass | 64,149 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda |