| IED ID | IndEnz0010000750 |
| Enzyme Type ID | esterase000750 |
| Protein Name |
Pectinesterase/pectinesterase inhibitor Includes: Pectinesterase inhibitor Pectin methylesterase inhibitor ; Pectinesterase PE EC 3.1.1.11 Pectin methylesterase |
| Gene Name | |
| Organism | Ficus pumila var. awkeotsang (Jelly fig) (Ficus awkeotsang) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Rosales Moraceae Ficus (figs) Ficus pumila (creeping fig) Ficus pumila var. awkeotsang (Jelly fig) (Ficus awkeotsang) |
| Enzyme Sequence | MEINQPNLLEASKSCYSKITFFLLVISFAALVSTGFSSPELSLHHKICDQSVNKESCLAMISEVTGLNMADHRNLLKSFLEKTTPRIQKAFETANDASRRINNPQERTALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLEEGSTDYIKTLMESHLNELILRARTSLAIFVTLFPAKSNVIEPVTGNFPTWVTAGDRRLLQTLGKDIEPDIVVAKDGSGDYETLNEAVAAIPDNSKKRVIVLVRTGIYEENVDFGYQKKNVMLVGEGMDYTIITGSRNVVDGSTTFDSATVAAVGDGFIAQDICFQNTAGPEKYQAVALRIGADETVINRCRIDAYQDTLYPHNYRQFYRDRNITGTVDFIFGNAAVVFQNCNLIPRKQMKGQENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESYISDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAELIQGGSWLGSTGVDYTAGLYA |
| Enzyme Length | 545 |
| Uniprot Accession Number | P83947 |
| Absorption | |
| Active Site | ACT_SITE 360; /note=Proton donor; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 381; /note=Nucleophile; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040 |
| Activity Regulation | |
| Binding Site | BINDING 307; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 337; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 449; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 451; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; Evidence={ECO:0000269|PubMed:11982420, ECO:0000269|PubMed:16667199}; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Disulfide bond (2); Glycosylation (2); Propeptide (1); Region (2); Sequence conflict (2); Signal peptide (1); Site (1) |
| Keywords | Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000269|PubMed:11982420}. |
| Signal Peptide | SIGNAL 1..37; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 60,602 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda |