Detail Information for IndEnz0010000752
IED ID IndEnz0010000752
Enzyme Type ID esterase000752
Protein Name Probable pectinesterase/pectinesterase inhibitor 20
Includes: Pectinesterase inhibitor 20
Pectin methylesterase inhibitor 20
; Pectinesterase 20
PE 20
EC 3.1.1.11
Pectin methylesterase 20
AtPME20
Gene Name PME20 ARATH20 At2g47550 T30B22.15
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MSQKLMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA
Enzyme Length 560
Uniprot Accession Number O22256
Absorption
Active Site ACT_SITE 376; /note=Proton donor; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 397; /note=Nucleophile; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040
Activity Regulation
Binding Site BINDING 323; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 353; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 465; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 467; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Binding site (4); Chain (1); Disulfide bond (1); Frameshift (1); Glycosylation (12); Region (3); Signal peptide (1); Site (1)
Keywords Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..24; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12509334; 12913156; 14585825; 15047898; 15235117; 15894741; 16766693; 17172353; 18192438; 19269997; 27247031; 28082716;
Motif
Gene Encoded By
Mass 61,497
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda