| IED ID | IndEnz0010000766 |
| Enzyme Type ID | esterase000766 |
| Protein Name |
Pectinesterase 31 PE 31 EC 3.1.1.11 Pectin methylesterase 31 AtPME31 |
| Gene Name | PME31 ARATH31 At3g29090 MXE2.5 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAGISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHIHCKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMDACIRNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQDRPWLCLRMGVKTPYSA |
| Enzyme Length | 317 |
| Uniprot Accession Number | Q9LVQ0 |
| Absorption | |
| Active Site | ACT_SITE 144; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 165; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040 |
| Activity Regulation | ACTIVITY REGULATION: Does not require salt for activity. Not inhibited by kiwi pectin methylesterase inhibitor (PMEI). {ECO:0000269|PubMed:18936961}. |
| Binding Site | BINDING 91; /note=Substrate; /evidence=ECO:0000250; BINDING 121; /note=Substrate; /evidence=ECO:0000250; BINDING 222; /note=Substrate; /evidence=ECO:0000250; BINDING 224; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; Evidence={ECO:0000269|PubMed:18936961}; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin (PubMed:18936961). Acts in a blockwise manner, resulting in a cell wall rigidification. {ECO:0000250, ECO:0000269|PubMed:18936961}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Fully active at 0 or 30 degrees Celsius, inactive at 55 degrees Celsius. {ECO:0000269|PubMed:18936961}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0-8.5. {ECO:0000269|PubMed:18936961}; |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Site (1) |
| Keywords | Aspartyl esterase;Hydrolase;Reference proteome |
| Interact With | Q93ZX1 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18805951; 21798944; 24367018; 27605050; 29307824; 32771166; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,520 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda | 3.1.1.11; |