Detail Information for IndEnz0010000769
IED ID IndEnz0010000769
Enzyme Type ID esterase000769
Protein Name Probable pectinesterase/pectinesterase inhibitor 34
Includes: Pectinesterase inhibitor 34
Pectin methylesterase inhibitor 34
; Pectinesterase 34
PE 34
EC 3.1.1.11
Pectin methylesterase 34
AtPME34
Gene Name PME34 ARATH34 At3g49220 F2K15.80
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MGYERLGPSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLSI
Enzyme Length 598
Uniprot Accession Number Q9M3B0
Absorption
Active Site ACT_SITE 413; /note=Proton donor; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 434; /note=Nucleophile; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040
Activity Regulation
Binding Site BINDING 360; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 390; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 502; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 504; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Alternative sequence (2); Binding site (4); Chain (1); Compositional bias (1); Disulfide bond (1); Erroneous initiation (1); Region (3); Site (1); Transmembrane (1)
Keywords Alternative splicing;Aspartyl esterase;Cell wall biogenesis/degradation;Disulfide bond;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 15272873; 15494554; 16367961; 17435085; 18577427; 18650403; 18805951; 21245191; 22253603; 27247031; 28082716; 28381503; 28617153;
Motif
Gene Encoded By
Mass 65,307
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda 3.1.1.11;