Detail Information for IndEnz0010000770
IED ID IndEnz0010000770
Enzyme Type ID esterase000770
Protein Name Probable pectinesterase/pectinesterase inhibitor 35
Includes: Pectinesterase inhibitor 35
Pectin methylesterase inhibitor 35
; Pectinesterase 35
PE 35
EC 3.1.1.11
Pectin methylesterase 35
AtPME35
Gene Name PME35 ARATH35 At3g59010 F17J16.60
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MATTSFSLPNHKFGIKLMLFLVLNLLSLQTSVFAHSSNSKFTKISRHPNSDSSSRTKPSTSSNKGFLSSVQLSLDHALFARSLAFNLTLSHRTSQTLMLDPVNDCLELLDDTLDMLYRIVVIKRKDHVNDDVHTWLSAALTNQETCKQSLSEKSSFNKEGIAIDSFARNLTGLLTNSLDMFVSDKQKSSSSSNLTGGRKLLSDHDFPTWVSSSDRKLLEASVEELRPHAVVAADGSGTHMSVAEALASLEKGSGRSVIHLTAGTYKENLNIPSKQKNVMLVGDGKGKTVIVGSRSNRGGWNTYQSATVAAMGDGFIARDITFVNSAGPNSEQAVALRVGSDRSVVYRCSIDGYQDSLYTLSKRQFYRETDITGTVDFIFGNSAVVFQSCNLVSRKGSSDQNYVTAQGRSDPNQNTGISIHNCRITGSTKTYLGRPWKQYSRTVVMQSFIDGSIHPSGWSPWSSNFALKTLYYGEFGNSGPGSSVSGRVSWAGYHPALTLTEAQGFTVSGFIDGNSWLPSTGVVFDSGLL
Enzyme Length 529
Uniprot Accession Number Q9LYT5
Absorption
Active Site ACT_SITE 355; /note=Proton donor; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 376; /note=Nucleophile; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040
Activity Regulation
Binding Site BINDING 302; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 332; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 434; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 436; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Binding site (4); Chain (1); Glycosylation (3); Region (3); Signal peptide (1); Site (1)
Keywords Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..34; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 15276459; 17013613; 18723362; 18805951; 19582540; 22693281;
Motif
Gene Encoded By
Mass 57,513
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda