Detail Information for IndEnz0010000777
IED ID IndEnz0010000777
Enzyme Type ID esterase000777
Protein Name Lipid droplet-associated hydrolase
EC 3.1.1.-
Lipid droplet-associated serine hydrolase
Gene Name sturkopf CG9186
Organism Drosophila melanogaster (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly)
Enzyme Sequence MQEAYVNINSIPTHIFTWGRWIEETITEKEIVICITGNPGLPGFYTEFAGTLQKELGDLPVWVIGHAGHDDPPEASIREVPQLSGNEELFNLDGQIRHKIAFIEKYVPSDVKIHLIGHSIGAWMILQLLENERIRSRIQKCYMLFPTVERMMESPNGWVFTKVAMPLYSVFGYIFFSFFNFLPVWLRLMLIQIYFLIFSIPRQFLGTALKYSKPSVAEKVVFLADDEMARVRGIQREIVEQNLDLLKFYYGTTDGWVPISYYDQLKKDYPKVDAQLDTKKIDHAFVLRHSQPMAVIVRDMIQQHRRV
Enzyme Length 307
Uniprot Accession Number Q9W0H3
Absorption
Active Site ACT_SITE 119; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 254; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9; ACT_SITE 283; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Probable serine lipid hydrolase associated with lipid droplets (By similarity). Appears to lack cholesterol esterase activity (PubMed:27836991). Appears to lack triglyceride lipase activity (PubMed:23525007, PubMed:27836991). Involved in negatively regulating juvenile hormone (JH) and possibly, insulin signaling activities such as triacylglycerols (TAG) storage, and thereby plays a role in the endocrine regulation of organismal growth and survival (PubMed:30917324). Likely functions by enhancing the activity of the JH hydrolase enzymes Jheh1 and Jheh2 (PubMed:30917324). Required for lipid droplet positioning and fat storage (PubMed:23525007). {ECO:0000250|UniProtKB:Q8BVA5, ECO:0000269|PubMed:23525007, ECO:0000269|PubMed:27836991, ECO:0000269|PubMed:30917324}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Region (1); Transmembrane (2)
Keywords Endoplasmic reticulum;Hydrolase;Lipid droplet;Membrane;Reference proteome;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:23525007, ECO:0000269|PubMed:27836991}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:23525007}; Multi-pass membrane protein {ECO:0000255}. Note=Localizes to the endoplasmic reticulum in absence of lipid droplets and translocates to lipid droplets upon lipid storage induction. {ECO:0000269|PubMed:23525007}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16783380; 16979555; 18245369; 18419806; 18783608; 19317464; 20813047; 23071443; 23319140; 23765091; 25312911; 26212136; 27197209; 27894253; 27956707; 28742022; 31722958; 32335968; 32730754; 32903680; 33827210; 33945525; 34993199;
Motif
Gene Encoded By
Mass 35,736
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda