| IED ID | IndEnz0010000788 |
| Enzyme Type ID | esterase000788 |
| Protein Name |
Perforin-1 P1 Cytolysin Lymphocyte pore-forming protein |
| Gene Name | Prf1 Pfp |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MATCLFLLGLFLLLPRPVPAPCYTATRSECKQKHKFVPGVWMAGEGMDVTTLRRSGSFPVNTQRFLRPDRTCTLCKNSLMRDATQRLPVAITHWRPHSSHCQRNVAAAKVHSTEGVAREAAANINNDWRVGLDVNPRPEANMRASVAGSHSKVANFAAEKTYQDQYNFNSDTVECRMYSFRLVQKPPLHLDFKKALRALPRNFNSSTEHAYHRLISSYGTHFITAVDLGGRISVLTALRTCQLTLNGLTADEVGDCLNVEAQVSIGAQASVSSEYKACEEKKKQHKMATSFHQTYRERHVEVLGGPLDSTHDLLFGNQATPEQFSTWTASLPSNPGLVDYSLEPLHTLLEEQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSCQCECQDSKVTNQDCCPRQRGLAHLVVSNFRAEHLWGDYTTATDAYLKVFFGGQEFRTGVVWNNNNPRWTDKMDFENVLLSTGGPLRVQVWDADYGWDDDLLGSCDRSPHSGFHEVTCELNHGRVKFSYHAKCLPHLTGGTCLEYAPQGLLGDPPGNRSGAVW |
| Enzyme Length | 554 |
| Uniprot Accession Number | P10820 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells (PubMed:19446473, PubMed:21037563, PubMed:3261391, PubMed:7526382, PubMed:8164737). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:19446473, PubMed:21037563, PubMed:3261391, PubMed:7526382, PubMed:8164737). Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes (PubMed:19446473, PubMed:21037563, PubMed:3261391, PubMed:7526382, PubMed:8164737). {ECO:0000269|PubMed:19446473, ECO:0000269|PubMed:21037563, ECO:0000269|PubMed:3261391, ECO:0000269|PubMed:7526382, ECO:0000269|PubMed:8164737}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (25); Chain (1); Disulfide bond (9); Domain (3); Glycosylation (3); Helix (15); Metal binding (6); Mutagenesis (4); Sequence conflict (6); Signal peptide (1); Site (2); Turn (8) |
| Keywords | 3D-structure;Calcium;Cell membrane;Cytolysis;Direct protein sequencing;Disulfide bond;EGF-like domain;Endosome;Glycoprotein;Lysosome;Membrane;Metal-binding;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane beta strand |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytolytic granule {ECO:0000269|PubMed:2040805, ECO:0000269|PubMed:8164737}. Secreted {ECO:0000269|PubMed:2040805, ECO:0000269|PubMed:3261391}. Cell membrane {ECO:0000269|PubMed:19446473, ECO:0000269|PubMed:21037563}; Multi-pass membrane protein {ECO:0000269|PubMed:19446473, ECO:0000269|PubMed:21037563}. Endosome lumen {ECO:0000250|UniProtKB:P14222}. Note=Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell (PubMed:2040805, PubMed:8164737). May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes (By similarity). Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change (PubMed:19446473, PubMed:21037563). {ECO:0000250|UniProtKB:P14222, ECO:0000269|PubMed:19446473, ECO:0000269|PubMed:2040805, ECO:0000269|PubMed:21037563, ECO:0000269|PubMed:8164737}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. The glycosylation sites are facing the interior of the pore. {ECO:0000269|PubMed:21037563}. |
| Signal Peptide | SIGNAL 1..20; /evidence="ECO:0000269|PubMed:3190678, ECO:0000269|PubMed:3261391" |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 3NSJ; 4Y1S; 4Y1T; 5UG6; 5UG7; |
| Mapped Pubmed ID | 10049941; 10072530; 10201999; 10207172; 10229850; 10352283; 10367905; 10395644; 10415034; 10430631; 10441734; 10452979; 10486155; 10510093; 10544197; 10570179; 10570301; 10587362; 10590269; 10631568; 10639149; 10684858; 10706715; 10779789; 10820381; 10843700; 10878371; 10946317; 11006223; 11046015; 11046034; 11082062; 11090183; 11133760; 11160245; 11333140; 11342601; 11380680; 11433343; 11438167; 11441070; 11497079; 11507223; 11559824; 11714837; 11739503; 11748177; 11752172; 11801676; 11856751; 11870623; 11884544; 11907109; 11911476; 12039912; 12084043; 12093877; 12147624; 12171798; 12239309; 12352968; 12387738; 12391238; 12517799; 12574320; 12574396; 12594834; 12601154; 12610115; 12616497; 12626579; 12648455; 12668139; 12705850; 12817750; 12847210; 12847225; 12874215; 12925844; 12960267; 14530349; 14557749; 14583233; 14688317; 14734739; 14742542; 14747539; 14764669; 15007091; 1505959; 15061770; 15069016; 15070687; 15084164; 15098030; 15114672; 15215168; 15265903; 15271900; 15308121; 15322178; 15322205; 15452725; 15454490; 15466930; 15492848; 15499555; 15534000; 15536146; 15567431; 15596864; 15599322; 15699103; 15755897; 15761851; 1577064; 15778381; 15879127; 15899830; 15944263; 15956596; 15963482; 15967825; 15969448; 15987490; 15998831; 16002721; 16019602; 16081783; 16116214; 16129707; 16155578; 16236268; 16237054; 16339537; 16339567; 16365400; 16373503; 16385625; 16414999; 16418326; 16436955; 16497935; 16539627; 16541469; 16709800; 16775321; 16869802; 16879622; 16888019; 16914553; 16920924; 16936188; 16966495; 16982903; 16997856; 17088087; 17112732; 17114464; 17197428; 17244679; 17259968; 17343612; 17475875; 17475905; 17509909; 17537992; 17540585; 17545627; 17562770; 17591955; 17599099; 17615584; 17675510; 17827390; 17919943; 17954569; 17982459; 18000953; 18064039; 18096765; 18227174; 18259609; 18413767; 18424703; 18469826; 18505782; 18563383; 18617557; 18632965; 18644885; 18768900; 18991284; 19005414; 19009526; 19017970; 19047627; 19050276; 19050279; 19084435; 19109156; 19119023; 19120481; 19139191; 19147124; 19196996; 19216020; 19262044; 19264954; 19290021; 19325035; 19338979; 19359516; 19406986; 19451396; 19525969; 19564888; 19602595; 19680139; 19720840; 19785028; 1981053; 19843883; 19878357; 19901080; 19903852; 19915045; 19923462; 20007464; 20008292; 20008293; 20035050; 20042681; 20048124; 20080755; 20083666; 20089706; 20097864; 20101613; 20156971; 20200356; 20215523; 20220087; 20233969; 20309009; 20357252; 20381385; 20404142; 20424188; 20439728; 20440077; 20483732; 20488796; 20497254; 20525896; 20570944; 20583106; 20585036; 20660713; 20814579; 20844537; 20935648; 2095003; 20951068; 21045195; 21135172; 21245496; 21257961; 21267068; 21278341; 21282337; 21296981; 21297009; 21304931; 21311565; 21357263; 21450826; 21525386; 21536799; 21606480; 21624938; 21658975; 21709155; 21712396; 21768400; 21832159; 21839735; 21853094; 21873193; 21878672; 21931672; 21964024; 21966366; 22031758; 22042696; 22075702; 22084442; 22101430; 22161269; 22167808; 22212899; 22216008; 22237627; 22250087; 22312128; 22366582; 22374983; 22491469; 22493083; 22532799; 22617213; 22674984; 22678903; 22751116; 22773667; 22776817; 22815291; 22844498; 22851709; 22869906; 22905147; 23002042; 23160464; 23164664; 23190531; 23209317; 23230000; 23275602; 23341634; 23355740; 23395974; 23402528; 23403622; 23440410; 23454771; 23568450; 23585675; 23660960; 23676498; 23691028; 23744295; 23746652; 23776508; 23840635; 23874205; 23883515; 23897612; 23964122; 23974195; 23999498; 24043891; 24061165; 24070258; 24096050; 24194600; 24205352; 24211274; 24259502; 24293627; 24366614; 24391492; 24415778; 24477907; 24487434; 24533050; 24550298; 24558045; 24586745; 24625974; 24658838; 24687086; 24698324; 24790144; 24854422; 24855001; 24906149; 25048196; 25337791; 25351955; 25352130; 25398236; 25428875; 25436971; 25459639; 25502180; 25525117; 25555453; 25750028; 25809566; 25902529; 25990242; 26066976; 26104914; 26306037; 26321222; 26384546; 26390156; 26426795; 26446877; 26447219; 26454821; 26467188; 26506472; 26518437; 26572063; 26588782; 26610869; 26720279; 26752517; 26755706; 26756195; 26806130; 26825707; 26864340; 26872694; 27268148; 27341123; 27377586; 27403737; 27608599; 27663893; 27737881; 27827367; 27856754; 28034913; 28096300; 28108629; 28137589; 28228283; 28233863; 28251796; 28264905; 28302645; 28533414; 28537251; 28860338; 29053971; 29293660; 29416110; 29420734; 29436413; 29695416; 29743559; 29891681; 29940333; 29975766; 30135490; 30301918; 30442932; 30575733; 30578871; 30745322; 30879886; 30894275; 31012985; 31015190; 31241463; 31243088; 31301754; 31400073; 31509742; 31572388; 31742873; 31924474; 32052406; 32152316; 32153566; 32180773; 32188940; 32286313; 32419134; 32528465; 32581358; 32726632; 32734619; 32925169; 32973802; 33188072; 33361812; 34547143; 34721405; 7494105; 7518614; 7520535; 7533086; 7533644; 7543509; 7558020; 7608538; 7734048; 7835907; 7959748; 7970703; 7972104; 8188645; 8344349; 8370390; 8570653; 8676085; 8835541; 8920866; 8977187; 8985346; 8990386; 9112346; 9259590; 9314549; 9362539; 9414161; 9464800; 9480986; 9506704; 9510164; 9521083; 9551899; 9551927; 9605173; 9681998; 9708802; 9736651; 9743374; 9820556; 9846702; 9862744; 9882377; 9916723; 9916752; |
| Motif | |
| Gene Encoded By | |
| Mass | 62,081 |
| Kinetics | |
| Metal Binding | METAL 435; /note=Calcium 1; /evidence=ECO:0000269|PubMed:21037563; METAL 483; /note=Calcium 1; /evidence=ECO:0000269|PubMed:21037563; METAL 484; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000269|PubMed:21037563; METAL 485; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000269|PubMed:21037563; METAL 488; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000269|PubMed:21037563; METAL 490; /note=Calcium 2; /evidence=ECO:0000269|PubMed:21037563 |
| Rhea ID | |
| Cross Reference Brenda |