| IED ID | IndEnz0010000801 |
| Enzyme Type ID | esterase000801 |
| Protein Name |
Neutral cholesterol ester hydrolase 1 NCEH EC 3.1.1.- Acetylalkylglycerol acetylhydrolase 2-acetyl MAGE hydrolase EC 3.1.1.71 Arylacetamide deacetylase-like 1 |
| Gene Name | NCEH1 AADACL1 KIAA1363 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MRSSCVLLTALVALAAYYVYIPLPGSVSDPWKLMLLDATFRGAQQVSNLIHYLGLSHHLLALNFIIVSFGKKSAWSSAQVKVTDTDFDGVEVRVFEGPPKPEEPLKRSVVYIHGGGWALASAKIRYYDELCTAMAEELNAVIVSIEYRLVPKVYFPEQIHDVVRATKYFLKPEVLQKYMVDPGRICISGDSAGGNLAAALGQQFTQDASLKNKLKLQALIYPVLQALDFNTPSYQQNVNTPILPRYVMVKYWVDYFKGNYDFVQAMIVNNHTSLDVEEAAAVRARLNWTSLLPASFTKNYKPVVQTTGNARIVQELPQLLDARSAPLIADQAVLQLLPKTYILTCEHDVLRDDGIMYAKRLESAGVEVTLDHFEDGFHGCMIFTSWPTNFSVGIRTRNSYIKWLDQNL |
| Enzyme Length | 408 |
| Uniprot Accession Number | Q6PIU2 |
| Absorption | |
| Active Site | ACT_SITE 191; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038; ACT_SITE 348; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038; ACT_SITE 378; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-O-alkyl-2-acetyl-sn-glycerol + H2O = 1-O-alkyl-sn-glycerol + acetate + H(+); Xref=Rhea:RHEA:11552, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15850, ChEBI:CHEBI:16291, ChEBI:CHEBI:30089; EC=3.1.1.71; Evidence={ECO:0000269|PubMed:17052608};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11553; Evidence={ECO:0000305|PubMed:17052608}; CATALYTIC ACTIVITY: Reaction=1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn-glycerol + acetate + H(+); Xref=Rhea:RHEA:38563, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:34115, ChEBI:CHEBI:75936; Evidence={ECO:0000269|PubMed:17052608};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38564; Evidence={ECO:0000305|PubMed:17052608}; CATALYTIC ACTIVITY: Reaction=a cholesterol ester + H2O = a fatty acid + cholesterol + H(+); Xref=Rhea:RHEA:36403, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:17002, ChEBI:CHEBI:28868; Evidence={ECO:0000250|UniProtKB:Q8BLF1};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36404; Evidence={ECO:0000250|UniProtKB:Q8BLF1}; CATALYTIC ACTIVITY: Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, ChEBI:CHEBI:46898; Evidence={ECO:0000250|UniProtKB:Q8BLF1};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; Evidence={ECO:0000250|UniProtKB:Q8BLF1}; |
| DNA Binding | |
| EC Number | 3.1.1.-; 3.1.1.71 |
| Enzyme Function | FUNCTION: Hydrolyzes 2-acetyl monoalkylglycerol ether (1-O-alkyl-2-acetyl-sn-glycerol), the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (PubMed:17052608). May be responsible for the hydrolysis of cholesterol esters (such as cholesteryl (9Z-octadecenoate)) in macrophages (By similarity). Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds (By similarity). May contribute to cancer pathogenesis by promoting tumor cell migration (PubMed:17052608). {ECO:0000250|UniProtKB:Q8BLF1, ECO:0000269|PubMed:17052608}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (2); Chain (1); Erroneous initiation (8); Glycosylation (3); Motif (1); Natural variant (3); Sequence conflict (6); Topological domain (2); Transmembrane (1) |
| Keywords | Alternative splicing;Cell membrane;Endoplasmic reticulum;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Microsome;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17052608, ECO:0000305|PubMed:12149457}; Single-pass type II membrane protein {ECO:0000305|PubMed:12149457}. Microsome {ECO:0000250|UniProtKB:Q8BLF1}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000269|PubMed:12149457, ECO:0000269|PubMed:19159218}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17052604; 19592704; 19851296; 20198315; 20217867; 20353815; 20947831; 23993462; 24868095; 25280398; 26496610; 26617293; 27015452; 28934392; 32321446; 33219617; |
| Motif | MOTIF 113..115; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
| Gene Encoded By | |
| Mass | 45,808 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:11552; RHEA:11553; RHEA:38563; RHEA:38564; RHEA:36403; RHEA:36404; RHEA:33875; RHEA:33876 |
| Cross Reference Brenda | 3.1.1.13; |