IED ID | IndEnz0010000808 |
Enzyme Type ID | esterase000808 |
Protein Name |
Carboxylesterase Culp6 homolog EC 3.1.1.- |
Gene Name | Cgl2873 |
Organism | Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Corynebacteriaceae Corynebacterium Corynebacterium glutamicum (Brevibacterium saccharolyticum) Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) |
Enzyme Sequence | MRKTITVIAVLIVLALIGVGIVQYVNTSDDSDFIGQPGEPTGTETTEPPVQPDWCPAVEVIAAPGTWESAANDDPINPTANPLSFMLSITQPLQERYSADDVKVWTLPYTAQFRNINSQNEMSYDDSRNEGTAKMNEELINTHNECPATEFIIVGFSQGAVIAGDVAAQIGSEQGVIPADSVRGVALIADGRREPGVGQFPGTFVDGIGAEVTLQPLNLLVQPIVPGATMRGGRAGGFGVLNDRVQDICAPNDAICDAPVNVGNALDRALAMVSANGVHALYATNPDVFPGTTTNAWVVDWATNLIDNG |
Enzyme Length | 309 |
Uniprot Accession Number | Q8NLR5 |
Absorption | |
Active Site | ACT_SITE 157; /evidence=ECO:0000250|UniProtKB:O53581; ACT_SITE 253; /evidence=ECO:0000250|UniProtKB:O53581; ACT_SITE 279; /evidence=ECO:0000250|UniProtKB:O53581 |
Activity Regulation | ACTIVITY REGULATION: Inhibited by tetrahydrolipstatin (THL), a specific lipase inhibitor. {ECO:0000269|PubMed:20656688}. |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+); Xref=Rhea:RHEA:47348, ChEBI:CHEBI:2571, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:50477; Evidence={ECO:0000269|PubMed:20656688}; |
DNA Binding | |
EC Number | 3.1.1.- |
Enzyme Function | FUNCTION: Esterase that may be involved in cell wall biosynthesis and/or maintenance. Hydrolyzes pNP-butyrate (C4). {ECO:0000269|PubMed:20656688}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Chain (1); Disulfide bond (2); Transmembrane (1) |
Keywords | Cell membrane;Disulfide bond;Hydrolase;Membrane;Reference proteome;Serine esterase;Transmembrane;Transmembrane helix |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000255}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 32,616 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.71 mM for pNP-butyrate {ECO:0000269|PubMed:20656688}; Vmax=273 nmol/min/mg enzyme with pNP-butyrate as substrate {ECO:0000269|PubMed:20656688}; Note=kcat is 0.146 sec(-1) with pNP-butyrate as substrate. {ECO:0000269|PubMed:20656688}; |
Metal Binding | |
Rhea ID | RHEA:47348 |
Cross Reference Brenda |