Detail Information for IndEnz0010000815
IED ID IndEnz0010000815
Enzyme Type ID esterase000815
Protein Name Pyrethroid hydrolase Ces2e
EC 3.1.1.88
Carboxylic ester hydrolase
EC 3.1.1.-
carboxylesterase 2E
Gene Name Ces2e Ces5
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MPLYKLLGWLNAVACGVLLLVLHVQGQDSASPIRNTHTGQVRGSLVHVKDTDIAVHTFLGIPFAKPPVGPLRFAPPEAPEPWSGVRDGTSHPNMCLQNDNLMGSEDLKMMNLILPPISMSEDCLYLNIYVPAHAHEGSNLPVMVWIHGGALTVGMASMYDGSMLAATEDVVVVAIQYRLGVLGFFSTGDQHAKGNWGYLDQVAALRWVQQNIVHFGGNPDRVTIFGESAGGTSVSSHVVSPMSQGLFHGAIMESGVAVLPDLISSSSEMVHRIVANLSGCAAVNSETLMCCLRGKNEAEMLAINKVFKIIPGVVDGEFLPKHPQELMASKDFHPVPSIIGINNDEYGWILPTIMDPAQKIEEITRKTLPAVLKSTALKMMLPPECGDLLMEEYMGDTEDPETLQAQFREMKGDFMFVIPALQVAHFQRSHAPVYFYEFQHRPSFFKDFRPPYVKADHGDEIFLVFGYQFGNIKLPYTEEEEQLSRRIMKYWANFARHGNPNSEGLPYWPVMDHDEQYLQLDIQPSVGRALKARRLQFWTKTLPQKIQELKGSQERHKEL
Enzyme Length 559
Uniprot Accession Number Q8BK48
Absorption
Active Site ACT_SITE 228; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 345; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23141; ACT_SITE 457; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23141
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(-)-trans-permethrin + H2O = (1S,3R)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate + (3-phenoxyphenyl)methanol + H(+); Xref=Rhea:RHEA:30283, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:62523, ChEBI:CHEBI:62527, ChEBI:CHEBI:62531; EC=3.1.1.88; Evidence={ECO:0000269|PubMed:15123619}; CATALYTIC ACTIVITY: Reaction=all-trans-retinyl hexadecanoate + H2O = all-trans-retinol + H(+) + hexadecanoate; Xref=Rhea:RHEA:13933, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336, ChEBI:CHEBI:17616; Evidence={ECO:0000250|UniProtKB:G3V7J5};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13934; Evidence={ECO:0000250|UniProtKB:G3V7J5};
DNA Binding
EC Number 3.1.1.88; 3.1.1.-
Enzyme Function FUNCTION: Carboxylesterase that catalyzes the hydrolysis of pyrethroids pesticides. Hydrolyzes trans-permethrin at a rate about 22-fold higher than cis-permethrin. Also hydrolyzes trans-cypermethrin. Hydrolyzes retinyl esters (By similarity). {ECO:0000250|UniProtKB:G3V7J5, ECO:0000269|PubMed:15123619}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (2); Glycosylation (1); Modified residue (2); Sequence conflict (3); Signal peptide (1)
Keywords Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Microsome;Phosphoprotein;Pyrrolidone carboxylic acid;Reference proteome;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Microsome {ECO:0000269|PubMed:15123619}.
Modified Residue MOD_RES 27; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000250|UniProtKB:P14943; MOD_RES 552; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:17208939
Post Translational Modification PTM: Glycosylated. {ECO:0000269|PubMed:15123619}.
Signal Peptide SIGNAL 1..26; /evidence=ECO:0000269|PubMed:15123619
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12466851; 14681479; 16615898; 17205597; 20931200; 21267068; 21677750;
Motif
Gene Encoded By
Mass 62,318
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=286 uM for p-Nitrophenyl acetate {ECO:0000269|PubMed:15123619}; KM=0.5 uM for cypermethrin {ECO:0000269|PubMed:15123619}; KM=2.2 uM for (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R/S)-trans/cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate {ECO:0000269|PubMed:15123619}; KM=0.89 uM for (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R)-(-)-2-(4-chlorophenyl)-3-methyl butanoate ((alphaR/S)(2R)-A3) {ECO:0000269|PubMed:15123619}; KM=0.96 uM for chrysanthemic acid {ECO:0000269|PubMed:15123619}; Note=kcat is 94 sec(-1) with p-Nitrophenyl acetate as substrate. kcat is 0.12 sec(-1) with cypermethrin as substrate. kcat is 0.11 sec(-1) with (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R/S)-trans/cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate as substrate. kcat is 0.067 sec(-1) with (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R)-(-)-2-(4-chlorophenyl)-3-methyl butanoate ((alphaR/S)(2R)-A3) as substrate. kcat is 0.099 sec(-1) with chrysanthemic acid as substrate.;
Metal Binding
Rhea ID RHEA:30283; RHEA:13933; RHEA:13934
Cross Reference Brenda 3.1.1.1;