IED ID | IndEnz0010000819 |
Enzyme Type ID | esterase000819 |
Protein Name |
Carboxylesterase 2 EC 3.1.1.1 Esterase II |
Gene Name | estB |
Organism | Pseudomonas fluorescens |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas fluorescens group (fluorescent pseudomonads) Pseudomonas fluorescens |
Enzyme Sequence | MTEPLILQPAKPADACVIWLHGLGADRYDFMPVAEALQESLLTTRFVLPQAPTRPVTINGGYEMPSWYDIKAMSPARSISLEELEVSAKMVTDLIEAQKRTGIDASRIFLAGFSQGGAVVFHTAFINWQGPLGGVIALSTYAPTFGDELELSASQQRIPALCLHGQYDDVVQNAMGRSAFEHLKSRGVTVTWQEYPMGHEVLPQEIHDIGAWLAARLG |
Enzyme Length | 218 |
Uniprot Accession Number | Q53547 |
Absorption | |
Active Site | ACT_SITE 114; /note=Charge relay system; /evidence=ECO:0000305|PubMed:9438866; ACT_SITE 168; /note=Charge relay system; /evidence=ECO:0000305|PubMed:9438866; ACT_SITE 199; /note=Charge relay system; /evidence=ECO:0000305|PubMed:9438866 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; |
DNA Binding | |
EC Number | 3.1.1.1 |
Enzyme Function | FUNCTION: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Beta strand (13); Chain (1); Helix (8); Turn (2) |
Keywords | 3D-structure;Direct protein sequencing;Hydrolase;Serine esterase |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (2) |
Cross Reference PDB | 1AUO; 1AUR; |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 23,880 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:21164 |
Cross Reference Brenda |