| IED ID | IndEnz0010000820 |
| Enzyme Type ID | esterase000820 |
| Protein Name |
Cocaine esterase EC 3.1.1.84 Liver carboxylesterase 2 EC 3.1.1.1 |
| Gene Name | CES2 ICE |
| Organism | Oryctolagus cuniculus (Rabbit) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Lagomorpha Leporidae (rabbits and hares) Oryctolagus Oryctolagus cuniculus (Rabbit) |
| Enzyme Sequence | QDSASPIRNTHTGQVRGSLVHVEGTDAGVHTFLGIPFAKPPLGPLRFAPPEPAEAWSGVRDGTSLPAMCLQNLAIMDQDVLLLHFTPPSIPMSEDCLYLNIYSPAHAREGSDLPVMVWIHGGGLTMGMASMYDGSALAAFEDVVVVTIQYRLGVLGFFSTGDQHATGNHGYLDQVAALRWVQKNIAHFGGNPGRVTIFGESAGGTSVSSHVLSPMSQGLFHGAIMESLVALLPGLITSSSEVVSTVVANLSRCGQVDSETLVRCLRAKSEEEMLAITQVFMLIPGVVDGVFLPRHPEELLALADFQPVPSIIGINNDEYGWIIPKLLLAIDPQEERDRQAMREIMHQATKQLMLPPALGDLLMDEYMGSNEDPKHLMAQFQEMMADAMFVMPALRVAHLQRSHAPTYFYEFQHRPSFTKDLRPPHVRADHGDEVVFVFRSHLFGSKVPLTEEEELLSRRVMKYWANFARNRNPNGEGLAHWPLFDLDQRYLQLNMQPAVGQALKARRLQFWTHTLPQRVQELRGTEQKHTEL |
| Enzyme Length | 532 |
| Uniprot Accession Number | P14943 |
| Absorption | |
| Active Site | ACT_SITE 201; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 318; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23141; ACT_SITE 430; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23141 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039}; CATALYTIC ACTIVITY: Reaction=cocaine + H2O = benzoate + ecgonine methyl ester + H(+); Xref=Rhea:RHEA:27506, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16150, ChEBI:CHEBI:59908, ChEBI:CHEBI:60056; EC=3.1.1.84; Evidence={ECO:0000250|UniProtKB:O00748}; CATALYTIC ACTIVITY: Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+); Xref=Rhea:RHEA:26132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:32395, ChEBI:CHEBI:52392; Evidence={ECO:0000250|UniProtKB:O00748};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:26133; Evidence={ECO:0000250|UniProtKB:O00748}; CATALYTIC ACTIVITY: Reaction=H2O + prostaglandin E2 1-glyceryl ester = glycerol + H(+) + prostaglandin E2; Xref=Rhea:RHEA:48296, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:90230, ChEBI:CHEBI:606564; Evidence={ECO:0000250|UniProtKB:O00748};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:48297; Evidence={ECO:0000250|UniProtKB:O00748}; CATALYTIC ACTIVITY: Reaction=H2O + prostaglandin F2alpha 1-glyceryl ester = glycerol + H(+) + prostaglandin F2alpha; Xref=Rhea:RHEA:48300, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:57404, ChEBI:CHEBI:90233; Evidence={ECO:0000250|UniProtKB:O00748};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:48301; Evidence={ECO:0000250|UniProtKB:O00748}; |
| DNA Binding | |
| EC Number | 3.1.1.84; 3.1.1.1 |
| Enzyme Function | FUNCTION: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (By similarity). {ECO:0000250|UniProtKB:O00748}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Glycosylation (1); Modified residue (1); Motif (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Lipid metabolism;Pyrrolidone carboxylic acid;Reference proteome;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000250|UniProtKB:O00748}. |
| Modified Residue | MOD_RES 1; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:2745458 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 529..532; /note=Prevents secretion from ER; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 59,059 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21164; RHEA:27506; RHEA:26132; RHEA:26133; RHEA:48296; RHEA:48297; RHEA:48300; RHEA:48301 |
| Cross Reference Brenda |