| IED ID | IndEnz0010000833 |
| Enzyme Type ID | esterase000833 |
| Protein Name |
Carboxylesterase 5A EC 3.1.1.1 Carboxylesterase-like urinary excreted protein homolog Cauxin Epididymis-specific gene 615 protein |
| Gene Name | Ces5a Ces7 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MSGDWVHTGQALIWVLWIFAAIIRGSVTEEPHRYTRLGWVQGKQATVLGRLEPVNVFLGIPFAAPPLGPLRFSNPQPPIPWHDLREATTYPNVCFQNLEWLFIYQNLLKVHYPKLGVSEDCLYLNIYAPAYANDGSRLPVMMWIPGGGFETGSASIFDGSALAAYEDVLIVTIQYRLGIFGFFNTQNQHAPGNWAFQDQLAALQWVRENINYFGGNPDSVTIFGGSAGAISISSLILSPLSAGLFHRAIMQSGVAIIPSLKNFDDELKHGLQVVADVCKCNVSDSKVLLKCLREKSSLELLSLGQKTKAFTRVVDGSFFPEEPMELLSQKTFKTVPSIIGVNNQECGYILPMREAPEILFGSNESTALTLIHVLLHIPPQYMHIVAKDYFHGKHSLTDIRDTLLDLFGDVFFVVPGLVTARNHRDADGPVYFYEFQHRPNCFQNTRPAFVKADHTDEIRFVFGGPFLEGDVVMFEEATEDEKLLSRKMMSYWANFARSGDPNGDDLPLWPAYDQNESYLKLDVNISTGWRLKDRRVEFWTDTLPLIMSASKALLSPTFPLILFSLLPPSLLSIAS |
| Enzyme Length | 575 |
| Uniprot Accession Number | Q5GRG2 |
| Absorption | |
| Active Site | ACT_SITE 226; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 345; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 454; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039}; |
| DNA Binding | |
| EC Number | 3.1.1.1 |
| Enzyme Function | FUNCTION: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.2. {ECO:0000269|PubMed:19727521}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Glycosylation (4); Signal peptide (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 25475668; |
| Motif | |
| Gene Encoded By | |
| Mass | 64,417 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=670 uM for p-NPA {ECO:0000269|PubMed:19727521}; Vmax=200 umol/min/mg enzyme with p-NPA as substrate {ECO:0000269|PubMed:19727521}; |
| Metal Binding | |
| Rhea ID | RHEA:21164 |
| Cross Reference Brenda |