Detail Information for IndEnz0010000837
IED ID IndEnz0010000837
Enzyme Type ID esterase000837
Protein Name Esterase-5B
Est-5B
EC 3.1.1.1
Carboxylic-ester hydrolase 5B
Carboxylesterase-5B
Gene Name Est-5B Est5b GA14349
Organism Drosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora obscura group pseudoobscura subgroup Drosophila pseudoobscura Drosophila pseudoobscura pseudoobscura (Fruit fly)
Enzyme Sequence MYCAKLILLLGCFWISSSASDPADPLLVDLPNGKLRGRDNGNYYSYESLPYAEPPVGDLRFEAPQPYKQQWTDTFDATQPPVLCMQWDQFIQGDDKLAGNEDCLTVSVYKPKNSSRNSFPVVAQIHGGAFMFGGASQNGHENFMREGNLILVKISYRLGPLGFVSTGDADLSGNFGLKDQRLALLWIKQNIASFGGEPENILVIGHSAGGGSVHLQVLREDFSKLAKAAISFSGNALDPWVVQQGGRGRAFELGRIVGCGQASDSVTLKKCLKSKPASEIVSAVRNFLVFAYVPFTPFGPVVESPDAPEAFISQHPVDIIKSGKFAQVPWAVTYTTEDGGYNAALLLEKQASSGRELIVDLNDRWFDWAPYLLFYRDSMTTIKDMDDYSRKLRQEYLGDRRFSVESYWDLQRLFTDVLFKNSTEISLDLHRKHGKSPVYAFVYDNPANTGIGQGLAKRTDINFGTVHGDDYFLIFENIVREPQLRSDEEIISRNFLKMLNDFVLSENGTLAFGTCVFQDNVGSSKLQLLSITRNGCENLELESFP
Enzyme Length 545
Uniprot Accession Number P25726
Absorption
Active Site ACT_SITE 207; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 467; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
DNA Binding
EC Number 3.1.1.1
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (3); Glycosylation (3); Natural variant (33); Sequence conflict (1); Signal peptide (1)
Keywords Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 60,652
Kinetics
Metal Binding
Rhea ID RHEA:21164
Cross Reference Brenda