| IED ID | IndEnz0010000849 |
| Enzyme Type ID | esterase000849 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 PICST_33358 |
| Organism | Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Debaryomycetaceae Scheffersomyces Scheffersomyces stipitis (Yeast) (Pichia stipitis) Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) |
| Enzyme Sequence | MKDSTEALNSIAFAVDTTLSSILPSSLAPPSAPPATSSFLKSIWYAFWWLWSMVVFKIMNIILLYIPSKIMNALSINFEITLNLSSILVALSAIITVCFLVVRYKYLTGYSKDTSDRKTKGKVNPSASNINLKNQSLDYVDQKKGHRRTTNYLDEFLSAIKVFGYLEKSVFHELTKNMTTQKLSHDEILYLDEKLGFSIVVEGVMQVYTRITENSNVSGGFDPDDDNELNYEKDDVLIIGNQRYQLLNEVKSGAPLSSLVSVLDLLKPVDSEDSTSDMLHSFNISDDDNISKIPEISPISLPFQGIHAGAKDDSVPPSPIIRPSKTKQLYPEIVARPKSRPHKEHTGHHLHHVHHSGATIAIIPYSAFQRVQSKYPKATSHIVTMVLARLYKVTFNTIHDYLGLTKEIMESEVKLNTTSSVRGANLPGYLFDGLLDKIYGANGINEASLSRKSDFQRASSVNLNKQKTTLCDAKSSRYVLLDSRLKSTHPGDLLSSVPLSRRSDYYQTHSHPLSADDPLVRSAFPSSKTLSSLSSPTSNLKRASSNLKFENIRDRSFSDDREETEETSLRIAVVESIFKILGISEKSTAMRNLSSFNSGRSSVSSSIVGLSNLMSADDKFDTNAARVRFDSYNGFSSTATSISRSSTPIKFYNTINQNQLHNHHMGDSVSGINISTLSRQHQANRNSSPTEFNFANVKSDFAKCLEIKSYGPNTTIVEQGSFNSGLYYVIDGSLDVLYRPSNHGEPSSNREDNLKKLYSVKSGGVAGYLSSVVGFRSLVTIRTSKKRGVIVAHISKSDYSKLMDRYYFLQLPVATKLKKLLSPQILTIDYALEWCHIPAGGVLCSQGDLANGFHIVLSGRFRVVRNKSDRYQGNTSDDDILGFSDTSMDCSPSSDINNEDLEVLGEYGHGESIGEVEVLTASRRTNSLIAVRDSETARIPRTLFEMLSLSNPSIMVKVSRIVASKVVYKDVLDQSSRNSTLIPSSTASHISNDYKTITILPTVSGLPVREFADKLVSALKAIGRNVIALDQASTLTHLGRHAFDERLAQLKLSGYFAYLEEEYETIVYICDTPLKSNWTSTCISQGDCILLLADAEDDVVATGIGDYERLLINLKTMARTDLCLLHPEKYVEPGSTSIWLKNRIWVQGHHHIEMEIIRKKDENSVKKRPNIISELASKIGSKTNPSIKSTLEDVRLKAISSFVKLNTSFVHSDRYKAVQPHKNDFLRLARILSNEAVGLVLGGGGSRGISHVGIVTALERHGIPVDLIGGTSIGSLVGGLYAKDYNIVSIYGRAKKFSKRVASLWRSVFDLTYPVTSYITGYEFNRGIWKIFGFTEIEDFWIRYFCNTTNITNSTMDIHESGYSWRFIRASMSLAGLLPPIAFQGCMLLDGGYLDNLPVSEMKKKGAKYIIAVDVGSADDRTPMNYGDTLSGFWVLFNRWNPFSKHPNVPNMMDIQMRLAYVASVNALEAAKKTNGVIYLRPPIDNYATLDFAKFDEIYHVGLNYADKLFSSWSKNGKLPAIAGMVDKAKIKSGDDKKVLYRRNSI |
| Enzyme Length | 1546 |
| Uniprot Accession Number | A3LYZ4 |
| Absorption | |
| Active Site | ACT_SITE 1272; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1390; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 689..820; /note=cNMP 1; NP_BIND 816..965; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Domain (1); Motif (3); Nucleotide binding (2); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1243..1248; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1270..1274; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1390..1392; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 171,904 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |