| IED ID | IndEnz0010000850 |
| Enzyme Type ID | esterase000850 |
| Protein Name |
Protein phosphatase methylesterase 1 PME-1 EC 3.1.1.89 |
| Gene Name | PPE1 KLLA0D13046g |
| Organism | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Kluyveromyces Kluyveromyces lactis (Yeast) (Candida sphaerica) Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) |
| Enzyme Sequence | MSGNLQREALLKEFENAGKMIHAFDTAPEGNGEEDTITELPVVKNPGLGVSSETKAENMEDTGFPRWTEFFAKNETVSLPNRNFTFNTYFQVPEVNSSDLSSIPIFLAHHGAGSTGLTFAPLAKTLKEDLGTNFGFFSFDARGHGETKPLDPTDVTYYLNDFVTDFVELIVWFYENYLKACKQSKLSLILVGHSLGGSVCANLYEHLPEYIKRHTTGLAMLDIVEEMAKFVLTKVDHFLSVTPNVFGSAKEAVDWYQSHNYSKLKESAEISVPALFHKAKSGKVVRITNLASFKPYWDSWFDGLSAKFVSLPTSKLLILAGNDNLDKELIVGQMQGKYQLIVFQDSGHFIQEDVPKKAAISLVDFWKRSDNRNVTIKTNWGSSQNKTLEGKKTVE |
| Enzyme Length | 395 |
| Uniprot Accession Number | Q6CQZ5 |
| Absorption | |
| Active Site | ACT_SITE 194; /evidence=ECO:0000250; ACT_SITE 222; /evidence=ECO:0000250; ACT_SITE 348; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + H(+) + methanol; Xref=Rhea:RHEA:48548, Rhea:RHEA-COMP:12134, Rhea:RHEA-COMP:12135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:90516, ChEBI:CHEBI:90517; EC=3.1.1.89; |
| DNA Binding | |
| EC Number | 3.1.1.89 |
| Enzyme Function | FUNCTION: Demethylates proteins that have been reversibly carboxymethylated. Demethylates the phosphatase PP2A catalytic subunit (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1) |
| Keywords | Hydrolase;Reference proteome;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 44,181 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:48548 |
| Cross Reference Brenda |