IED ID | IndEnz0010000852 |
Enzyme Type ID | esterase000852 |
Protein Name |
Protein phosphatase methylesterase 1 PME-1 EC 3.1.1.89 Pectin methylesterase 1 |
Gene Name | pme-1 ppe1 B20J13.050 NCU00954 |
Organism | Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Sordariaceae Neurospora Neurospora crassa Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
Enzyme Sequence | MSELQKQWAKARLGSQALGQPPAEMPITTTMPEFNEADEDKEMAGPSPGGFPFDDDSSSASSVSSTGTVIPSPGRALFARPQGFASRSMDPIPWTTYFERELFLKEEAGPDSGSRTSNKNKPTSITYHAYLTSPVGKGPLFVTHHGAGSSGLSFAVLSSEIRKRLPNAGILSLDARGHGSTTVTAASSVPGEGDETTGQAPPPLDLSLSTLASDLFTVIQLTRTAMHWPELPPIILVGHSLGGAVVTELAKSYRLGPSLLGYAVLDVVEGCAMDALQSMQTYLSTRPQGFASLKDGIDWHVRSRTIRNSTSARTSVPGLLAPVEELQRPELQQLPRGVAGTQGTAKPWRWKTDLAATQPFWEDWFVGLSKKFLEARGGKMLLLAGTDRLDTELTIGQMQGKYALQVFPEAGHFIHEDLPEKTAIALVDFHRRNDRSQLVLPPKVSDLLAQGKKV |
Enzyme Length | 454 |
Uniprot Accession Number | Q7SGG8 |
Absorption | |
Active Site | ACT_SITE 240; /evidence=ECO:0000250; ACT_SITE 266; /evidence=ECO:0000250; ACT_SITE 412; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + H(+) + methanol; Xref=Rhea:RHEA:48548, Rhea:RHEA-COMP:12134, Rhea:RHEA-COMP:12135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:90516, ChEBI:CHEBI:90517; EC=3.1.1.89; |
DNA Binding | |
EC Number | 3.1.1.89 |
Enzyme Function | FUNCTION: Demethylates proteins that have been reversibly carboxymethylated. Demethylates the phosphatase PP2A catalytic subunit (By similarity). {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Chain (1); Compositional bias (1); Erroneous initiation (1); Region (2) |
Keywords | Hydrolase;Reference proteome;Serine esterase |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 48,955 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:48548 |
Cross Reference Brenda |