Detail Information for IndEnz0010000860
IED ID IndEnz0010000860
Enzyme Type ID esterase000860
Protein Name Carboxylesterase Culp6 homolog
EC 3.1.1.-
Gene Name MSMEG_6394 MSMEI_6226
Organism Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycolicibacterium Mycolicibacterium smegmatis (Mycobacterium smegmatis) Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Enzyme Sequence MAKNARRKRHRILALIAAAAMALVVVLVVTIVVVIMRRPDTPATPPPSAEPPGGVVVPPGTRKPRPEFQSADCPDVMMVSIPGTWESSPTDDPFNPTQFPLSLMSNISKPLAEQFGPDRLQVYTTPYTAQFHNPFAADKQMSYNDSRAEGMRTTVKAMTDMNDRCPLTSYVIAGFSQGAVIAGDIASDIGNGRGPVDEDLVLGVTLIADGRRQMGVGQDVGPNPAGQGAEITLHEVPALSALGLTMTGPRPGGFGALDNRTNQICGSGDLICSAPEQAFSVFNLPKTLETLSGSAAGPVHALYNTPQFWVENGQTATQWTLEWARNLVENAPHPKHG
Enzyme Length 337
Uniprot Accession Number A0R619
Absorption
Active Site ACT_SITE 176; /evidence=ECO:0000305|PubMed:20656688; ACT_SITE 269; /evidence=ECO:0000305|PubMed:20656688; ACT_SITE 300; /evidence=ECO:0000305|PubMed:20656688
Activity Regulation ACTIVITY REGULATION: Inhibited by tetrahydrolipstatin (THL), a specific lipase inhibitor. {ECO:0000269|PubMed:20656688}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+); Xref=Rhea:RHEA:47348, ChEBI:CHEBI:2571, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:50477; Evidence={ECO:0000269|PubMed:20656688};
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Esterase that may be involved in cell wall biosynthesis and/or maintenance. Hydrolyzes pNP-butyrate (C4). {ECO:0000269|PubMed:20656688}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Beta strand (9); Chain (1); Compositional bias (1); Disulfide bond (2); Helix (10); Region (1); Transmembrane (1); Turn (6)
Keywords 3D-structure;Cell membrane;Disulfide bond;Hydrolase;Membrane;Reference proteome;Serine esterase;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000255}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 3AJA;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 35,888
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=5.22 mM for pNP-butyrate {ECO:0000269|PubMed:20656688}; Vmax=783 nmol/min/mg enzyme with pNP-butyrate as substrate {ECO:0000269|PubMed:20656688}; Note=kcat is 0.448 sec(-1) with pNP-butyrate as substrate. {ECO:0000269|PubMed:20656688};
Metal Binding
Rhea ID RHEA:47348
Cross Reference Brenda