| IED ID | IndEnz0010000867 |
| Enzyme Type ID | esterase000867 |
| Protein Name |
Pectinesterase/pectinesterase inhibitor 3 Includes: Pectinesterase inhibitor 3 Pectin methylesterase inhibitor 3 ; Pectinesterase 3 PE 3 EC 3.1.1.11 Pectin methylesterase 27 AtPME27 Pectin methylesterase 3 |
| Gene Name | PME3 ARATH27 At3g14310 MLN21.10 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAPSMKEIFSKDNFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL |
| Enzyme Length | 592 |
| Uniprot Accession Number | O49006 |
| Absorption | |
| Active Site | ACT_SITE 409; /note=Proton donor; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 430; /note=Nucleophile; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040 |
| Activity Regulation | |
| Binding Site | BINDING 356; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 386; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 498; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 500; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Disulfide bond (1); Frameshift (1); Glycosylation (2); Region (2); Sequence caution (1); Sequence conflict (2); Signal peptide (1); Site (1) |
| Keywords | Apoplast;Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000269|PubMed:15593128}. Secreted, cell wall {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..35; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12566592; 12923061; 14576160; 14585825; 14973164; 16709196; 16776300; 16920880; 17277091; 17317660; 18252844; 18467450; 18538804; 18650403; 18796151; 18805951; 19001564; 21171891; 21692803; 22301970; 22319074; 23826687; 24367018; 24855660; 25128920; 25442819; 26183897; 28034952; 28082716; 28330559; 28375469; 29109147; 32344669; |
| Motif | |
| Gene Encoded By | |
| Mass | 64,256 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda | 3.1.1.11; |