Detail Information for IndEnz0010000869
IED ID IndEnz0010000869
Enzyme Type ID esterase000869
Protein Name Pectinesterase 3
PE 3
EC 3.1.1.11
Pectin methylesterase 3
Gene Name PME3
Organism Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum subgen. Lycopersicon Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Enzyme Sequence MATPLQPFLTKTHKQNPIIGFNILTFVVTLFVALFLVVFLVAPYQFEIKHSNLCKTAQDSQLCLSYVSEIVTTESDGVTVLKKFLVKYVHQMNNAIPVVRKIKNQINDIRQQGALTDCLELLDQSVDLVSDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELTSFSLSTKNGTVLDELITRAKVALAMLASVTTPNDEVLRQGLGKMPYWVSSRDRKLMESSGKDIIANRVVAQDGTGDYQTLAEAVAAAPDKNKTRYVIYVKMGIYKENVVVTKKKMNLMIVGDGMNATIITGSLNVVDGSTFPSNTLAAVGQGFILQDICIQNTAGPEKDQAVALRVGADMSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQIVARKPNKRQKNMVTAQGRTDPNQATGTSIQFCDIIASPDLEPVMNEYKTYLGRPWKKHSRTVVMQSYLDGHIDPSGWFEWRGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPNEAMPFTVAELIQGGSWLNSTSVAYVEGLVE
Enzyme Length 544
Uniprot Accession Number Q96576
Absorption
Active Site ACT_SITE 359; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 380; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040
Activity Regulation
Binding Site BINDING 306; /note=Substrate; /evidence=ECO:0000250; BINDING 336; /note=Substrate; /evidence=ECO:0000250; BINDING 448; /note=Substrate; /evidence=ECO:0000250; BINDING 450; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Binding site (4); Chain (1); Glycosylation (4); Signal peptide (1); Site (1)
Keywords Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Fruit ripening;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..?; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 60,249
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda