IED ID | IndEnz0010000876 |
Enzyme Type ID | esterase000876 |
Protein Name |
Probable pectinesterase/pectinesterase inhibitor 46 Includes: Pectinesterase inhibitor 46 Pectin methylesterase inhibitor 46 ; Pectinesterase 46 PE 46 EC 3.1.1.11 Pectin methylesterase 46 AtPME46 |
Gene Name | PME46 ARATH46 At5g04960 MUG13.18 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MSSYGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEPISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEHMDNATSAAMGACVELIGLAVDQLNETMTSSLKNFDDLRTWLSSVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNSDF |
Enzyme Length | 564 |
Uniprot Accession Number | Q9FF78 |
Absorption | |
Active Site | ACT_SITE 385; /note=Proton donor; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 406; /note=Nucleophile; for pectinesterase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040 |
Activity Regulation | |
Binding Site | BINDING 332; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 362; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 470; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 472; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250 |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; |
DNA Binding | |
EC Number | 3.1.1.11 |
Enzyme Function | FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
nucleotide Binding | |
Features | Active site (2); Binding site (4); Chain (1); Disulfide bond (1); Glycosylation (6); Region (2); Sequence conflict (2); Site (1); Transmembrane (1) |
Keywords | Aspartyl esterase;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 15634198; 16622707; 17408486; 17616511; 18178671; 18687588; 18725200; 22253603; 28181322; |
Motif | |
Gene Encoded By | |
Mass | 62,170 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:22380 |
Cross Reference Brenda |