Detail Information for IndEnz0010000882
IED ID IndEnz0010000882
Enzyme Type ID esterase000882
Protein Name Probable pectinesterase 55
PE 55
EC 3.1.1.11
Pectin methylesterase 55
AtPME55
Gene Name PME55 ARATH55 At5g18990 T16G12.30
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MGTHRIILGLAALCCFCLPHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVEDLPIRV
Enzyme Length 330
Uniprot Accession Number Q3E9D3
Absorption
Active Site ACT_SITE 161; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 182; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation
Binding Site BINDING 243; /note=Substrate; /evidence=ECO:0000250; BINDING 245; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Binding site (2); Chain (1); Glycosylation (4); Signal peptide (1); Site (1)
Keywords Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..24; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16299181; 16622707; 17915010; 20226671;
Motif
Gene Encoded By
Mass 36,460
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda