Detail Information for IndEnz0010000884
IED ID IndEnz0010000884
Enzyme Type ID esterase000884
Protein Name Putative pectinesterase 57
PE 57
EC 3.1.1.11
Pectin methylesterase 57
AtPME57
Gene Name PME57 ARATH57 At1g44980 F27F5.7
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MAMIGVMEDSKLHEDMENDMLGDLTSRAREALALFISISLRDNTELNLVVPNGPSWLSHVDKKDLYLNDETLKKITDILVAKDVTGNYNIVNVAIAAAPQHSQKRFVIYIKTSIYVEIVVIGNMKSNLTLIADGQDSTIITFNLSSSNSKRTFNTATFASNGDGFIRVDMCFRNTTWPVKGPVVTLRVNGDMSIIYRCRVEEYQDALYPHKNRQCYREYFLMDTVDFICGNAAAVFQFCQIVHMDG
Enzyme Length 246
Uniprot Accession Number Q9MAL0
Absorption
Active Site ACT_SITE 205; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 226; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation
Binding Site BINDING 152; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Binding site (1); Chain (1); Erroneous gene model prediction (1); Glycosylation (3); Site (1)
Keywords Aspartyl esterase;Glycoprotein;Hydrolase;Reference proteome
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16622707; 18936961; 23951288; 25572606;
Motif
Gene Encoded By
Mass 27,743
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda