| IED ID | IndEnz0010000885 |
| Enzyme Type ID | esterase000885 |
| Protein Name |
Pectinesterase QRT1 PE QRT1 EC 3.1.1.11 Pectin methylesterase 62 AtPME62 Pectin methylesterase QRT1 Protein QUARTET 1 AtQRT1 |
| Gene Name | QRT1 ARATH62 At5g55590 MDF20.3 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MKVEAFIPAVLLLCFGVMLCLKSSCALQIGNNNELKNYISWEDLRVVEDGRIERSFSIKENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL |
| Enzyme Length | 380 |
| Uniprot Accession Number | Q9FM79 |
| Absorption | |
| Active Site | ACT_SITE 221; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P0C1A9; ACT_SITE 242; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P0C1A9 |
| Activity Regulation | |
| Binding Site | BINDING 164; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1A9; BINDING 198; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1A9; BINDING 298; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1A9; BINDING 300; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1A9 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; Evidence={ECO:0000269|PubMed:16980565, ECO:0000269|PubMed:9744097}; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Pectinesterase required for cell type-specific pectin degradation to separate microspores. {ECO:0000269|PubMed:16980565, ECO:0000269|PubMed:8197459, ECO:0000269|PubMed:9744097}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. {ECO:0000269|PubMed:16980565, ECO:0000269|PubMed:9744097}. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Disulfide bond (1); Glycosylation (4); Signal peptide (1); Site (1) |
| Keywords | Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12805593; 16024587; 17360452; 18227644; 18492870; 20697737; 21140166; 22106370; 30333839; |
| Motif | |
| Gene Encoded By | |
| Mass | 42,475 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda |