Detail Information for IndEnz0010000886
IED ID IndEnz0010000886
Enzyme Type ID esterase000886
Protein Name Putative pectinesterase 63
PE 63
EC 3.1.1.11
Pectin methylesterase 63
AtPME63
Gene Name PME63 ARATH63 At5g61680 K11J9.6
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MGYNYVSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPPSF
Enzyme Length 338
Uniprot Accession Number Q9FKF3
Absorption
Active Site ACT_SITE 174; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 195; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation
Binding Site BINDING 116; /note=Substrate; /evidence=ECO:0000250; BINDING 151; /note=Substrate; /evidence=ECO:0000250; BINDING 252; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Binding site (3); Chain (1); Erroneous gene model prediction (1); Signal peptide (1); Site (1)
Keywords Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Hydrolase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..24; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16622707; 20032078; 20736450;
Motif
Gene Encoded By
Mass 37,324
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda