| IED ID | IndEnz0010000887 |
| Enzyme Type ID | esterase000887 |
| Protein Name |
Probable pectinesterase/pectinesterase inhibitor 64 Includes: Pectinesterase inhibitor 64 Pectin methylesterase inhibitor 64 ; Pectinesterase 64 PE 64 EC 3.1.1.11 Pectin methylesterase 64 AtPME64 |
| Gene Name | PME64 ARATH64 At5g64640 MUB3.16 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDSPTLPHSISASSSTPFASAAVKPHRNKLLSRNGILIIIAASCILLLLISLLIYATVSKSSRNHHNPSHQTPTSDDHPPPETPPSPPPIAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILDSSAGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLGSGTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSRVPWSSEIPEKHVDVYSVANFIQADEWASTTA |
| Enzyme Length | 602 |
| Uniprot Accession Number | Q8L7Q7 |
| Absorption | |
| Active Site | ACT_SITE 420; /note=Proton donor; for pectinesterase activity; /evidence=ECO:0000250; ACT_SITE 441; /note=Nucleophile; for pectinesterase activity; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 367; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 397; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 518; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250; BINDING 520; /note=Substrate; for pectinesterase activity; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Compositional bias (1); Disulfide bond (1); Erroneous initiation (1); Glycosylation (7); Region (3); Site (1); Transmembrane (1) |
| Keywords | Aspartyl esterase;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17317660; 18775970; 29284022; |
| Motif | |
| Gene Encoded By | |
| Mass | 65,478 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda | 3.1.1.11; |