| IED ID | IndEnz0010000888 |
| Enzyme Type ID | esterase000888 |
| Protein Name |
Probable pectinesterase 66 PE 66 EC 3.1.1.11 Pectin methylesterase 66 AtPME66 |
| Gene Name | PME66 ARATH66 At2g47280 T8I13.12 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MKRVASYNYKIVCMVVMTLFVYGYSAAAEQIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDGWISRFPIEGGP |
| Enzyme Length | 336 |
| Uniprot Accession Number | Q4PSQ5 |
| Absorption | |
| Active Site | ACT_SITE 164; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 185; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 247; /note=Substrate; /evidence=ECO:0000250; BINDING 249; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (2); Chain (1); Erroneous gene model prediction (1); Glycosylation (2); Signal peptide (1); Site (1) |
| Keywords | Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15258262; 16299181; 16622707; 17559508; 17915010; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,017 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda |