Detail Information for IndEnz0010000893
IED ID IndEnz0010000893
Enzyme Type ID esterase000893
Protein Name Pectinesterase
PE
EC 3.1.1.11
Pectin methylesterase
Gene Name pme1
Organism Aspergillus niger
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus niger
Enzyme Sequence MVKSILASVLFAATALAASRMTAPSGAIVVAKSGGDYDTISAAVDALSTTSTETQTIFIEEGSYDEQVYIPALSGKLIVYGQTEDTTTYTSNLVNITHAIALADVDNDDETATLRNYAEGSAIYNLNIANTCGQACHQALAVSAYASEQGYYACQFTGYQDTLLAETGYQVYAGTYIEGAVDFIFGQHARAWFHECDIRVLEGPSSASITANGRSSESDDSYYVIHKSTVAAADGNDVSSGTYYLGRPWSQYARVCFQKTSMTDVINHLGWTEWSTSTPNTENVTFVEYGNTGTGAEGPRANFSSELTEPITISWLLGSDWEDWVDTSYIN
Enzyme Length 331
Uniprot Accession Number P17872
Absorption
Active Site ACT_SITE 161; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 182; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040
Activity Regulation
Binding Site BINDING 138; /note=Substrate; /evidence=ECO:0000250; BINDING 247; /note=Substrate; /evidence=ECO:0000250; BINDING 249; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Involved in maceration and soft-rotting of plant tissue.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Binding site (3); Chain (1); Signal peptide (1); Site (1)
Keywords Aspartyl esterase;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..17
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 35,715
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda