Detail Information for IndEnz0010000897
IED ID IndEnz0010000897
Enzyme Type ID esterase000897
Protein Name Pectinesterase
PE
EC 3.1.1.11
Pectin methylesterase
Fragments
Gene Name
Organism Capsicum chinense (Scotch bonnet) (Bonnet pepper)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Capsiceae Capsicum (peppers) Capsicum chinense (Scotch bonnet) (Bonnet pepper)
Enzyme Sequence IDAFQDTLYTHTLRTYLGRPWK
Enzyme Length 22
Uniprot Accession Number P86085
Absorption
Active Site ACT_SITE 6; /note="Proton donor"; /evidence="ECO:0000250|UniProtKB:P83218, ECO:0000255|PROSITE-ProRule:PRU10040"
Activity Regulation
Binding Site BINDING 19; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P83218; BINDING 21; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P83218
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; Evidence={ECO:0000250|UniProtKB:P83218};
DNA Binding
EC Number 3.1.1.11
Enzyme Function
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (1); Binding site (2); Chain (1); Non-adjacent residues (1); Non-terminal residue (2); Sequence uncertainty (7); Site (1)
Keywords Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Secreted
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250|UniProtKB:P83218}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 2,696
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda