Detail Information for IndEnz0010000901
IED ID IndEnz0010000901
Enzyme Type ID esterase000901
Protein Name Lysosomal acid lipase/cholesteryl ester hydrolase
Acid cholesteryl ester hydrolase
LAL
EC 3.1.1.13
Cholesteryl esterase
Lipase A
Sterol esterase
Gene Name Lipa Lal Lip1
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MQLLGRVICFVVGILLSGGPTGTISAVDPEANMNVTEIIMHWGYPEHSVQTGDGYILGVHRIPHGRKNQFDKGPKPVVYLQWRHGFLADSSNWVTNIDNNSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEYWAFSFDEMAKYDLPASINYILNKTGQEQLYNVGHSQGCTIGFIAFSQMPELAKKVKMFFALAPVLSLNFASGPMVKLGRLPDLLLEDLFGQKQFLPQSAMVKWLSTHICTHVIMKELCANIFFLICGFNEKNLNMSRVDVYTTHCPAGTSVQNMVHWTQVVKYHKLQAFDWGSSDKNYFHYNQSYPPLYSIKDMQLPTALWSGGKDWLADTSDINILLTEIPTLVYHKNIPEWDHLDFIWGLDAPWRLYNEVVSLMKKYQ
Enzyme Length 397
Uniprot Accession Number Q64194
Absorption
Active Site ACT_SITE 172; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 372; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a sterol ester + H2O = a fatty acid + a sterol + H(+); Xref=Rhea:RHEA:10100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15889, ChEBI:CHEBI:28868, ChEBI:CHEBI:35915; EC=3.1.1.13; Evidence={ECO:0000250|UniProtKB:P38571}; CATALYTIC ACTIVITY: Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, ChEBI:CHEBI:46898; Evidence={ECO:0000250|UniProtKB:P38571};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; Evidence={ECO:0000250|UniProtKB:P38571};
DNA Binding
EC Number 3.1.1.13
Enzyme Function FUNCTION: Catalyzes the deacylation of triacylglyceryl and cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol. {ECO:0000250|UniProtKB:P38571}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Domain (1); Glycosylation (5); Propeptide (1); Signal peptide (1)
Keywords Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Lysosome;Reference proteome;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Lysosome {ECO:0000269|PubMed:4062953}.
Modified Residue
Post Translational Modification PTM: Glycosylation is not essential for catalytic activity. {ECO:0000250|UniProtKB:P38571}.
Signal Peptide SIGNAL 1..25; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10419291; 11687729;
Motif
Gene Encoded By
Mass 45,186
Kinetics
Metal Binding
Rhea ID RHEA:10100; RHEA:33875; RHEA:33876
Cross Reference Brenda