| IED ID | IndEnz0010000916 |
| Enzyme Type ID | esterase000916 |
| Protein Name |
Lysophospholipase nte1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | nte1 AN2481 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MADSGASVPSPDLPDLDSLHASLSLPDIPSVAATSLSTSLPAISAKVSGFPSFSSHLPPPPLLSPPTPTTMVGWIGWVFSLVFQTIPSVLYWVITFSTITLPTWLFTLFSMSLTFTMNFTTLLLIVLGLVSTVSWFIRYRFLNMYSRLPPEPQRKEPQLDLFPDVQEGDSKPGLANYLDEFLSAIKVFGYLERPVFHELTRTMQTRKLIAGETLQLEEEKGFCLVVDGLVQIFVKSMRSGKHGLNGEVIEGSSDEDDQARDGKQGYQLLTEVKNGASMSSLFSILSLFTEDIRLRASEGSSSSMSSVQPSPARTTPAPFLDSPGEMLNGSPMVLPRDSEVDSSAINGEAEPLPPVPPLHLGESRASSYHPNGQSTASERVRGNRRKSVHPDIVARAMVDTTIAIIPASAFRRLTRLYPRATAHIVQVILTRLQRVTFATAHSYLGLTNEVLGIEKQMTKFTTYDLPNDIRGAALDRLKDKFLKEKDRLGSEEVTRGIALHNPYAGRRRRSMSFVRKEAALKAKMPLPKRPNSLINPERPFHGYDTAGVSPGDLLSTIQLSRFGPRHDQFATTPRLHSPLTEKERSPLRRSSLQRKDSVDEDALFRESILDCIMKGIGLTPSSHNALRKGSHSGELSPKLVSYDSRRQKAVFSNNAFGFIDAYEGSGDGDTESMMSMSVTSAGGTSPIVYLREDLLNDIEIVYFPKGAVLVEQGERHPGLYYVIDGFLDVGVQVNEKGDDLVGASRPGHAQPDEELFPTLKRTQTATSRGATAAAPINESKRKKPSRKSLYMIKPGGMQGYVGAMASYRSYTDVVAKTDVYVGFLPRASLERLAERYPIALLTLAKRLTGLLPRLLLHIDFALEWVQVNAGQVIYHQGDESDAIYITLNGRLRSVHEGKGGKMTVVGEHGQGESVGELEVMTESTRPATLHAIRDTELAKFPRSLFNSLAQEHTGITIQVSKLIAQRMRDLVENPMTEQGEPGNTGSVKTATSTLNLRTVGILPITTGVPVVEFGNRLLSALQQIGVTDGVTSLNQAAILNHLGRHAFSRMGKLKLSQYLADLEEKYGMVLYIADTNVNSPWTQTCISQADCILLVGLAESSPNVGEYERFLLGMKTTARKELVLLHADRYCPPGLTRKWLKNRVWINGGHHHIQMAFRLTAEPSHPQTKRLGTVLKQRVQILQAEIQKYTSRRIRQTPIYSAQTPFKGDFHRLARRLCGRAVGLVLGGGGARGIAHVGVIKALEEAGIPVDIVGGTSIGAFIGGLYARDADVVPMYGRAKKFAGRMGSIWRFALDLTYPSVSYTTGHEFNRGIFKTFGDSQIEDFWLEFYCNTTNISRSRAEYHSSGYVWRYVRASMSLAGLLPPICDEGSMLLDGGYIDNLTVAHMKTLGADVIFAIDVGSIDDNTPQGYGDSLSGMWSVINRWNPFSSIPNPPTLSEIQARLAYVSSIDNLERAKNIPGCLYMRPPIDRYGTLEFGNFDEIYQVGYAYGKEYLQKLKSQGSLPLPEENEEKKKLQRTLAPRRASI |
| Enzyme Length | 1527 |
| Uniprot Accession Number | Q5BAE9 |
| Absorption | |
| Active Site | ACT_SITE 1257; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1375; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 682..809; /note=cNMP 1; NP_BIND 846..966; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (2); Domain (1); Erroneous gene model prediction (2); Motif (3); Nucleotide binding (2); Region (4); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1228..1233; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1255..1259; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1375..1377; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 168,413 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |