IED ID | IndEnz0010000956 |
Enzyme Type ID | esterase000956 |
Protein Name |
HTH-type transcriptional regulator GbdR Glycine betaine/dimethylglycine responsive regulator |
Gene Name | gbdR PA5380 |
Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Enzyme Sequence | MTTYAPGVPPQNRNPQSIGFLLLDNFTLISLASAVEPLRMANQLSGRELYRWHTLSLDGRQVWASDGLQITPDAGTDNAPAVDCVIVCGGVGIQRSVTREHVTFLQAQARQGRRLGAVCTGSWALARAGLLDGYDCSVHWECLAAMQEAFPRVAMSTRLFSIDRNRFTSSGGTAPMDMMLHLIGREHGRELSAAISEMFIYERIRNEQDHQRVPLKHMLGTNQPKLQEIVALMEANLEEPIDLDELAVYVNVSRRQLERLFQKYLHCSPSRYYLKLRLIRARQLLKQTSMSIIEVASVCGFVSTPHFSKCYREYFGIPPRDERQGQPLGQPVVLMPIPQDLALMPNSSALSALSQAQGESTFASVRI |
Enzyme Length | 367 |
Uniprot Accession Number | Q9HTI4 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | DNA_BIND 244..265; /note=H-T-H motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU00593; DNA_BIND 292..315; /note=H-T-H motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU00593 |
EC Number | |
Enzyme Function | FUNCTION: Specific regulator of choline metabolism, which activates transcription of at least 25 genes from 11 promoters in response to choline metabolites (PubMed:24097953). Required for the induction of plcH, encoding the phospholipase C, and pchP, encoding the phosphorylcholine phosphatase, in response to glycine betaine (GB) and dimethylglycine (DMG) (PubMed:19103776). Also controls the expression of gbcAB and dgcAB, which are required for GB and DMG degradation, respectively, in response to both GB and DMG (PubMed:17951379). The GbdR regulon also includes genes encoding sarcosine, glycine and serine catabolic enzymes, the BetX and CbcXWV quaternary amine transport proteins and the acetylcholine esterase gene, choE (PubMed:24097953). Acts by binding directly to the promoter region of the genes (PubMed:19103776, PubMed:24097953). May play an important role during P.aeruginosa interactions with eukaryotes (PubMed:17951379). Is critical for induction of plcH and pchP in response to bovine surfactant and mouse bronchoalveolar lavage fluid (BALF) (PubMed:19103776). {ECO:0000269|PubMed:17951379, ECO:0000269|PubMed:19103776, ECO:0000269|PubMed:24097953}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Chain (1); DNA binding (2); Domain (1) |
Keywords | Activator;DNA-binding;Reference proteome;Transcription;Transcription regulation |
Interact With | |
Induction | INDUCTION: The highest level of transcriptional activity is reached in the presence of choline. Regulated at the transcriptional level by a sigma-54-dependent promoter. Transcription can be activated by NtrC in the absence of a preferential nitrogen source, by CbrB in the absence of a preferential carbon source, and by the integration host factor (IHF), by favouring DNA bending. Negatively regulated by BetI in the absence of choline. {ECO:0000269|PubMed:28791946}. |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 41,000 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |