| IED ID | IndEnz0010000956 |
| Enzyme Type ID | esterase000956 |
| Protein Name |
HTH-type transcriptional regulator GbdR Glycine betaine/dimethylglycine responsive regulator |
| Gene Name | gbdR PA5380 |
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Enzyme Sequence | MTTYAPGVPPQNRNPQSIGFLLLDNFTLISLASAVEPLRMANQLSGRELYRWHTLSLDGRQVWASDGLQITPDAGTDNAPAVDCVIVCGGVGIQRSVTREHVTFLQAQARQGRRLGAVCTGSWALARAGLLDGYDCSVHWECLAAMQEAFPRVAMSTRLFSIDRNRFTSSGGTAPMDMMLHLIGREHGRELSAAISEMFIYERIRNEQDHQRVPLKHMLGTNQPKLQEIVALMEANLEEPIDLDELAVYVNVSRRQLERLFQKYLHCSPSRYYLKLRLIRARQLLKQTSMSIIEVASVCGFVSTPHFSKCYREYFGIPPRDERQGQPLGQPVVLMPIPQDLALMPNSSALSALSQAQGESTFASVRI |
| Enzyme Length | 367 |
| Uniprot Accession Number | Q9HTI4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 244..265; /note=H-T-H motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU00593; DNA_BIND 292..315; /note=H-T-H motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU00593 |
| EC Number | |
| Enzyme Function | FUNCTION: Specific regulator of choline metabolism, which activates transcription of at least 25 genes from 11 promoters in response to choline metabolites (PubMed:24097953). Required for the induction of plcH, encoding the phospholipase C, and pchP, encoding the phosphorylcholine phosphatase, in response to glycine betaine (GB) and dimethylglycine (DMG) (PubMed:19103776). Also controls the expression of gbcAB and dgcAB, which are required for GB and DMG degradation, respectively, in response to both GB and DMG (PubMed:17951379). The GbdR regulon also includes genes encoding sarcosine, glycine and serine catabolic enzymes, the BetX and CbcXWV quaternary amine transport proteins and the acetylcholine esterase gene, choE (PubMed:24097953). Acts by binding directly to the promoter region of the genes (PubMed:19103776, PubMed:24097953). May play an important role during P.aeruginosa interactions with eukaryotes (PubMed:17951379). Is critical for induction of plcH and pchP in response to bovine surfactant and mouse bronchoalveolar lavage fluid (BALF) (PubMed:19103776). {ECO:0000269|PubMed:17951379, ECO:0000269|PubMed:19103776, ECO:0000269|PubMed:24097953}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); DNA binding (2); Domain (1) |
| Keywords | Activator;DNA-binding;Reference proteome;Transcription;Transcription regulation |
| Interact With | |
| Induction | INDUCTION: The highest level of transcriptional activity is reached in the presence of choline. Regulated at the transcriptional level by a sigma-54-dependent promoter. Transcription can be activated by NtrC in the absence of a preferential nitrogen source, by CbrB in the absence of a preferential carbon source, and by the integration host factor (IHF), by favouring DNA bending. Negatively regulated by BetI in the absence of choline. {ECO:0000269|PubMed:28791946}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 41,000 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |