Detail Information for IndEnz0010000978
IED ID IndEnz0010000978
Enzyme Type ID esterase000978
Protein Name Lovastatin esterase
PcEST
EC 3.1.1.-
Gene Name PcEST Pc15g00720 PCH_Pc15g00720
Organism Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Penicillium Penicillium chrysogenum species complex Penicillium rubens Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Enzyme Sequence MDTTFQAAIDTGKINGAVVCATDAQGHFVYNKATGERTLLSGEKQPQQLDDVLYLASATKLITTIAALQCVEDGLLSLDGDLSSIAPELAAKYVLTGFTDDESPLDDPPARPITLKMLLTHSSGTSYHFLDPSIAKWRAQYANPENEKPRLVEEMFTYPLSFQPGTGWMYGPGLDWAGRVVERVTGGTLMEFMQKRIFDPLGITDSQFYPVTREDLRARLVDLNPSDPGALGSAVIGGGGEMNLRGRGAFGGHGLFLTGLDFVKILRSLLANDGMLLKPAAVDNMFQQHLGPEAAASHRAALASPLGPFFRVGTDPETKVGYGLGGLLTLEDVDGWYGERTLTWGGGLTLTWFIDRKNNLCGVGAIQAVLPVDGDLMADLKQTFRHDIYRKYSAWKGQQ
Enzyme Length 399
Uniprot Accession Number B6H6L7
Absorption
Active Site ACT_SITE 57; /note=Nucleophile; /evidence=ECO:0000269|PubMed:31839596; ACT_SITE 60; /note=Proton acceptor; /evidence=ECO:0000269|PubMed:31839596; ACT_SITE 170; /note=Proton acceptor; /evidence=ECO:0000269|PubMed:31839596
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=H2O + lovastatin = (S)-2-methylbutanoate + H(+) + monacolin J; Xref=Rhea:RHEA:62748, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:40303, ChEBI:CHEBI:79034, ChEBI:CHEBI:145932; Evidence={ECO:0000269|PubMed:28619444};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62749; Evidence={ECO:0000269|PubMed:28619444}; CATALYTIC ACTIVITY: Reaction=H2O + pravastatin lactone = (S)-2-methylbutanoate + H(+) + pravastatin diol lactone; Xref=Rhea:RHEA:62752, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:145931, ChEBI:CHEBI:145932, ChEBI:CHEBI:145933; Evidence={ECO:0000269|PubMed:28619444};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62753; Evidence={ECO:0000269|PubMed:28619444}; CATALYTIC ACTIVITY: Reaction=H2O + mevastatin = (S)-2-methylbutanoate + compactin diol lactone + H(+); Xref=Rhea:RHEA:62744, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:34652, ChEBI:CHEBI:34848, ChEBI:CHEBI:145932; Evidence={ECO:0000269|PubMed:28619444};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62745; Evidence={ECO:0000269|PubMed:28619444};
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Esterase that can hydrolyze the side chain of lovastatin to produce monacolin J (PubMed:28619444, PubMed:31839596). Is also able to hydrolyze the side chains of mevastatin and pravastatin, but not simvastatin (PubMed:28619444). {ECO:0000269|PubMed:28619444, ECO:0000269|PubMed:31839596}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Beta strand (16); Chain (1); Helix (17); Mutagenesis (7); Turn (6)
Keywords 3D-structure;Hydrolase;Reference proteome
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (4)
Cross Reference PDB 6KJC; 6KJD; 6KJE; 6KJF;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 43,292
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=39.4 uM for lovastatin (at 37 degrees Celsius) {ECO:0000269|PubMed:28619444}; KM=5.46 uM for lovastatin (at 30 degrees Celsius) {ECO:0000269|PubMed:31839596};
Metal Binding
Rhea ID RHEA:62748; RHEA:62749; RHEA:62752; RHEA:62753; RHEA:62744; RHEA:62745
Cross Reference Brenda