Detail Information for IndEnz0010000992
IED ID IndEnz0010000992
Enzyme Type ID esterase000992
Protein Name Esterase mokD
EC 3.1.2.31
Monacolin K biosynthesis protein D
Gene Name mokD
Organism Monascus pilosus (Red mold)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Monascus Monascus pilosus (Red mold)
Enzyme Sequence MRIQRTPAPPKAPRALLCVHGAGCSPAIFRVQLSKLRAALREDFEFVYATAPFPSAPGPGILPTFEGLGPYYTWFEGSPSGAAAKGDNSNSNDSNSSPTVHDRLAAVHEPVRRAIAEWQTQNPSIPIVGTVSFSEGALVTALLLWQQQMGRIPWLPVMQVAMFICCWYQHEATQYMREEVGCGGDGGIDGEKLVIRGVLSLHLQGRDDFALAGSKMVVAQHFVPGEAQVLEFAGRHHFPNRPRDVLETVKRFRQLCVRARVIG
Enzyme Length 263
Uniprot Accession Number Q3S2U0
Absorption
Active Site ACT_SITE 134; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P38777; ACT_SITE 208; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P38777; ACT_SITE 236; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P38777
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=dihydromonacolin L-[lovastatin nonaketide synthase] + H2O = dihydromonacolin L carboxylate + H(+) + holo-[lovastatin nonaketide synthase]; Xref=Rhea:RHEA:11592, Rhea:RHEA-COMP:10042, Rhea:RHEA-COMP:10043, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:79031, ChEBI:CHEBI:79032; EC=3.1.2.31; Evidence={ECO:0000250|UniProtKB:Q0C8M2};
DNA Binding
EC Number 3.1.2.31
Enzyme Function FUNCTION: Esterase; part of the gene cluster that mediates the biosynthesis of monakolin K, also known as lovastatin, and which acts as a potent competitive inhibitor of HMG-CoA reductase (PubMed:18578535). Monakolin K biosynthesis is performed in two stages (PubMed:19693441). The first stage is catalyzed by the nonaketide synthase mokA, which belongs to type I polyketide synthases and catalyzes the iterative nine-step formation of the polyketide (PubMed:18578535, PubMed:19693441). This PKS stage completed by the action of dehydrogenase mokE, which catalyzes the NADPH-dependent reduction of the unsaturated tetra-, penta- and heptaketide intermediates that arise during the mokA-mediated biosynthesis of the nonaketide chain and leads to dihydromonacolin L (PubMed:19693441). Covalently bound dihydromonacolin L is released from mokA by the mokD esterase (By similarity). Conversion of dihydromonacolin L into monacolin L and then monacolin J is subsequently performed with the participation of molecular oxygen and P450 monoogygenase mokC (PubMed:19693441). Finally, mokF performs the conversion of monacoline J to monacoline K through the addition of the side-chain diketide moiety (2R)-2-methylbutanoate produced by the diketide synthase mokB (PubMed:19693441). {ECO:0000250|UniProtKB:Q0C8M2, ECO:0000269|PubMed:19693441, ECO:0000303|PubMed:18578535}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Polyketide biosynthesis; lovastatin biosynthesis. {ECO:0000250|UniProtKB:Q0C8M2}.
nucleotide Binding
Features Active site (3); Chain (1)
Keywords Hydrolase
Interact With
Induction INDUCTION: Expression is controlled by the monacolin K cluster transcription regulator mokH (PubMed:19968298). {ECO:0000269|PubMed:19968298}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 28,843
Kinetics
Metal Binding
Rhea ID RHEA:11592
Cross Reference Brenda