| IED ID | IndEnz0010001000 |
| Enzyme Type ID | esterase001000 |
| Protein Name |
Pectinesterase 5 PE 5 EC 3.1.1.11 Pectin methylesterase 5 AtPME5 Pectin methylesterase 67 AtPME67 Protein VANGUARD 1 |
| Gene Name | PME5 ARATH67 VGD1 At2g47040 F14M4.13 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MIGKVVVSVASILLIVGVAIGVVAYINKNGDANLSPQMKAVRGICEATSDKASCVKTLEPVKSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCKKVFMYALEDLSTIVEEMGEDLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDDLRKTIGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPPVADDSPVADPDGPARRLLEDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVVAKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVISYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIFNCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTADGNEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRPEGWKIWDGESFHKSCRYVEYNNRGPGAITNRRVNWVKIARSAAEVNDFTVANWLGPINWIQEANVPVTLGL |
| Enzyme Length | 595 |
| Uniprot Accession Number | Q5MFV8 |
| Absorption | |
| Active Site | ACT_SITE 413; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 434; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040 |
| Activity Regulation | |
| Binding Site | BINDING 360; /note=Substrate; /evidence=ECO:0000250; BINDING 390; /note=Substrate; /evidence=ECO:0000250; BINDING 503; /note=Substrate; /evidence=ECO:0000250; BINDING 505; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. Plays an important role in growth of pollen tubes in female floral tissues, possibly via enhancing the interaction between the pollen tube and female floral tissues by modification of the cell walls (PubMed:15659637). May be regulated by MYB80 during anther development and play a role in tapetum and pollen development (PubMed:21673079). {ECO:0000269|PubMed:15659637, ECO:0000269|PubMed:21673079}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Glycosylation (3); Motif (2); Region (1); Sequence conflict (7); Signal peptide (1); Site (1) |
| Keywords | Aspartyl esterase;Cell membrane;Cell wall;Cell wall biogenesis/degradation;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15659637}. Secreted, cell wall {ECO:0000269|PubMed:15659637}. Golgi apparatus membrane {ECO:0000269|PubMed:19144003}. Note=Distributed in the whole pollen tube, including the plasma membrane and pollen tube wall (PubMed:15659637). Cleaved in the Golgi apparatus by SBT6.1 (S1P) after the Arg-Arg-Leu-Leu (RRLL) and Arg-Lys-Leu-Met (RKLM) motifs. This processing is required for extracellular targeting (PubMed:19144003). {ECO:0000269|PubMed:15659637, ECO:0000269|PubMed:19144003}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15860015; 18502975; 18775970; 21278126; 21415319; 32817253; 9767082; |
| Motif | MOTIF 243..246; /note=RRLL cleavage motif; /evidence=ECO:0000305|PubMed:19144003; MOTIF 263..266; /note=RKLM cleavage motif; /evidence=ECO:0000305|PubMed:19144003 |
| Gene Encoded By | |
| Mass | 64,728 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda | 3.1.1.11; |