| IED ID | IndEnz0010001003 |
| Enzyme Type ID | esterase001003 |
| Protein Name |
Probable pectinesterase A EC 3.1.1.11 Pectin methylesterase A |
| Gene Name | pmeA AFUA_8G06880 |
| Organism | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Neosartorya fumigata (Aspergillus fumigatus) Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Enzyme Sequence | MYLPSLVLGLLGFGLTASTSPIEERSNRSKAPAGCLTVGSSGTYATIGAALSALGSSTSDACIFIGAGTYQEQITIDYKGKLTMYGETTDTSSYKQNLVTITHSISSPEAGSLDKSATVNVRSDGFKMYNINVINGYGKGAQAVTLVANADKLGFYGCSFVGYQDTLYAKAGRQYYSNCYIEGAVDYIFGDASAWFGECDLVSVGPGYITAMSRTTADETTWYAIDHCNIYGKPGVDLTSAVYLGRPWRVLARVIFQNSQLSNIINPKGWSPMATGATPLYYEYNNKGAGADTSKREYESPISGAVSIATVLGGGWNSWVDTTY |
| Enzyme Length | 324 |
| Uniprot Accession Number | Q4WBT5 |
| Absorption | |
| Active Site | ACT_SITE 165; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 186; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040 |
| Activity Regulation | |
| Binding Site | BINDING 142; /note=Substrate; /evidence=ECO:0000250; BINDING 246; /note=Substrate; /evidence=ECO:0000250; BINDING 248; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11; |
| DNA Binding | |
| EC Number | 3.1.1.11 |
| Enzyme Function | FUNCTION: Involved in maceration and soft-rotting of plant tissue. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| nucleotide Binding | |
| Features | Active site (2); Binding site (3); Chain (1); Glycosylation (1); Signal peptide (1); Site (1) |
| Keywords | Aspartyl esterase;Cell wall biogenesis/degradation;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 34,558 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22380 |
| Cross Reference Brenda |