| IED ID | IndEnz0010001020 |
| Enzyme Type ID | esterase001020 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 MGG_06143 |
| Organism | Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Magnaporthales Pyriculariaceae Pyricularia Magnaporthe oryzae (Rice blast fungus) (Pyricularia oryzae) Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
| Enzyme Sequence | MDSSTAALATASAKLDAVAQQGSSSWIGFFANIILGIISLVYSILYSVLKLTTFSIPSLLYTLFSTSLTVTMNATTLMLIIVLVFSLVSWFVRYRYLNMYSRLPPEPQRKEPDVDLFPDTHPGGSKPGLSNYLDEFLSAIKIFGYLERPVFHELTRSMQTRKLIAGETFNLEEEKGFCMVVDGLVEIFVKSARTGQHPVGESCSIDSDGSDEHEEYQAGNQRYQLLTEVHNGAPMSSLFSIMSLFTEDIKLRHGGDEGGEPTSATETYTSSRYGLGNDFSDQRESTVSVPTTPQLERSSSHGPTLHDGDANPSMGGRVPASIPPMSLDPPLQSRPKRPTTSRHATTSVHPDIIARATVDTTIAIIPASAFRRLIQIYPKYTAHIVHVILSRFQRVTLATAYNYLGLTSEVLHIEKSMIDNATCQLPNFLRGDALTRLKEKFNHERERIGEKDCTKGIALHNSSTGRRRKSTASLRKEAALQAITHGQRPSSVTASPPLQPRESGNKHTSNSGDLLMNIQLSRNGGRRSTSNMDPLTRQSTLLGSLETEVVSPLSQRTSNPFETRRHAHISLNKRETRDEDGLFRESILECMFKAIGLTANGTGAREPDSADASPRLTSYDQIRRGGYSSHNAFGFIDPYGGSVDGDADSITSGGTGPGLPVNPHALAHDMRDEVEIVFFPSGSVLVEQGERNPGLYYVVDGFLDVCMSAADESSGDILKASKNESSLNIGPGNQQGSAGRDRFMDANLGQSSSRKRASNVRRNVALIKPGGLAGYVGSISSHRSFIDVVAKTDVYVGFLPRQSLERIVDRYPIVLLTMAKRLTNLLPKLILHIDFALEWLQVNAGEVIFHGGEESEAIYIVLNGRLRLVEDRQEGGVDVRGEFGQGESIGELEVLTESARSGTLHAIRNTELVKFPRTLFNSLAQEHPNITIKISKIIASRMRKVVDDPSTFVGKEGALKASLNKSSSTLNLRTVAILPVTSGVPVVEFGNRLMGALTQVGTPNGATSLSQSAILNHLGRHAFNKMGKLKLSQYLADLEEKYGLVMYVADTNVNSPWTQTCITQADCILLVGLAEGSPEIGEYERFMLGMKSTARKVLVLLHADRYAEPGLTRSWLRNRMWINGGHHHVQMAFRTQSVPINPTAKRTGPSLKERVQILQAEIQKYTSRKVRHSPFYSPDAPFKGDFHRLARRLCGKSVGLVLGGGGARGLAHIGIIRAMEESGIPIDIVGGTSIGSFVGALYARHADVVPIFGLSKKFAGRMASVWRFALDLTYPSASYTTGHEFNRGIFKTFGKAQIEDFWLEFYCNTTNISKSRQEIHTSGYAWRYVRASMSLAGLLPPLCDEGSMLLDGGYIDNLTVSHMKSLGVDIIFAVDVGSLDDDVPQAYGDTLSGLWAFVNRWNPLSSTPNPPTLAEIQARLAYVSSVDALERAKMTPGCVYMRPPIDEYGTLEFGRFDEIVQVGYKYGLEFLQKLRDEGALPVVEETEAKKALRRTMAPRRASI |
| Enzyme Length | 1503 |
| Uniprot Accession Number | A4QVZ8 |
| Absorption | |
| Active Site | ACT_SITE 1233; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1351; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 658..777; /note=cNMP 1; NP_BIND 821..941; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (3); Domain (1); Motif (3); Nucleotide binding (2); Region (3); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1204..1209; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1231..1235; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1351..1353; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 165,037 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |