| IED ID | IndEnz0010001058 |
| Enzyme Type ID | esterase001058 |
| Protein Name |
Palmitoleoyl-protein carboxylesterase notum2 EC 3.1.1.98 |
| Gene Name | notum2 |
| Organism | Xenopus laevis (African clawed frog) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Xenopus Xenopus laevis (African clawed frog) |
| Enzyme Sequence | MRILEIFAILLILKEVRPQTKRSHKVNMKTPFQKRKNHHLNTDSRATLMSPADLHSVEHSIVQIRDPTLIPHYDQKSQEDMKLHFLKNTLVTCNDGTTAGYYLRETKGSKRWIIFLEGGWCCYSKETCGVRYDNVKRLMSSSNWPQTRKGTGILSPRQDENPYWWNVNAVFVPYCSSDVWSGNVSKTQDGYAFMGSVIIQEVIRDLVPRGMKQAKSVILAGSSAGGTGVLINIDRVAALVEETTSESVQVRGLVDSGWFLDSRHSKQSDCLDISKCALTEAIKKGLKLWNGILPENCKQQFKKGDEWRCFYGPRLFTSMKSPIFVVQWLYDQEQLRIENIQTEFQSMTENQWNSIQNIGREFKKSLREVPAVFAPACLSHTLITKSNWLEFQVKSVTLAKALHCWDRSLQENRAPKGVIRGCPFHLVDNCQWPHCNPTCPAIYDATSGQELSILQMFLKLRLESQRRGQEPKGDLGPLMSMLRNSG |
| Enzyme Length | 486 |
| Uniprot Accession Number | A0A0D3QS97 |
| Absorption | |
| Active Site | ACT_SITE 223; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 331; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988; ACT_SITE 380; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6P988 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + H2O = (9Z)-hexadecenoate + [Wnt protein]-L-serine + H(+); Xref=Rhea:RHEA:45340, Rhea:RHEA-COMP:11170, Rhea:RHEA-COMP:11171, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:32372, ChEBI:CHEBI:85189; EC=3.1.1.98; Evidence={ECO:0000250|UniProtKB:Q6P988}; |
| DNA Binding | |
| EC Number | 3.1.1.98 |
| Enzyme Function | FUNCTION: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleylation of WNT proteins. Serine palmitoleylation of WNT proteins is required for efficient binding to frizzled receptors. {ECO:0000250|UniProtKB:Q6P988}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (1); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal;Wnt signaling pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VUX3}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,452 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:45340 |
| Cross Reference Brenda |