Detail Information for IndEnz0010001062
IED ID IndEnz0010001062
Enzyme Type ID esterase001062
Protein Name Probable feruloyl esterase ARB_07085
EC 3.1.1.73
Gene Name ARB_07085
Organism Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Onygenales Arthrodermataceae (dermatophytes) Trichophyton Arthroderma benhamiae (Trichophyton mentagrophytes) Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes)
Enzyme Sequence MVTLPLLLSILPLAAVFSSAASLQVRENHDCASIQPPQVPGAEILSVIGVQRRVEVAPFPPSPSKPNTTIDICSVNVTLSHMGVNDKVVVSVWLPLPDKWNGRFQATGGGGWAAGTFDLLMGPAALEGYSTAGTDAGVTVDPGSADKWALKEDGTVNYDLLENFASRSIHDMAIVGKAVTESYYKKPANYSYFYGCSNGGRQGMVEAQKYPDDFDGILAGAPAIYWPQFLTSTEWPQVVMQSEKVFPSQCVFEAFRKAGIAACDKLDGVEDGVVSNLDGCEFNPFALVGKKVKCGEESTTITLAQAWVAKKIYDGPKSTAKHALWDVLPVGASYVGLANSTIENGVPKIAPFVIGSSWIRSFLKKDVNFDLSTITYADMPKLFQQSIDEFDKIAGGSNPDLSALKKSGTKLLSWHGLADELIHPQGSIKYRQAVEHRMGGGSEVDNYYRLFLAPGVTHCGIGVNDGAAPIDTLKVLVRWVEKGEAPETMPATATDASGTTTLFTRNLCRYPLVPRYKGGDKNSADSFECAKDFGSHH
Enzyme Length 537
Uniprot Accession Number D4AS70
Absorption
Active Site ACT_SITE 197; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q2UP89; ACT_SITE 419; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q2UP89; ACT_SITE 458; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q2UP89
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide.; EC=3.1.1.73; Evidence={ECO:0000250|UniProtKB:Q8WZI8};
DNA Binding
EC Number 3.1.1.73
Enzyme Function FUNCTION: Hydrolyzes the feruloyl-arabinose ester bond in arabinoxylans as well as the feruloyl-galactose and feruloyl-arabinose ester bonds. {ECO:0000250|UniProtKB:Q8WZI8}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Glycosylation (4); Metal binding (5); Signal peptide (1)
Keywords Calcium;Carbohydrate metabolism;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Secreted;Serine esterase;Signal;Xylan degradation
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21247460, ECO:0000269|PubMed:21919205}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..22; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 57,777
Kinetics
Metal Binding METAL 264; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 267; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 269; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 271; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q2UP89; METAL 273; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q2UP89
Rhea ID
Cross Reference Brenda